J Integr Plant Biol. ›› 2005, Vol. 47 ›› Issue (9): 1123-1132.DOI: 10.1111/j.1744-7909.2005.00119.x

• Research Articles • Previous Articles    

Cloning and Expression Pattern of a Gene Encoding a Putative Plastidic ATP/ADP Transporter from Helianthus tuberosus L.

Kun MENG, Tuan-Jie CHANG, Xiang LIU, Song-Biao CHEN, Yong-Qin WANG, Ai-Jun SUN, Hong-Lin XU, Xiao-Li WEI and Zhen ZHU   

  • Published:2005-09-01

Abstract: Herein, we report the cloning and molecular characterization of a full cDNA encoding a putative plastidic ATP/ADP transporter, designated HtAATP, for Helianthus tuberosus L. The ATP/ADP translocator protein was isolated from the tuber-cDNA library of H. tuberosus for the first time. The predicted HtAATP protein was judged as a plastidic ATP/ADP translocator protein from its high homology at the amino acid sequence level to the two Arabidopsis thaliana plastidic ATP/ADP translocator proteins AATP1 and AATP2 (84.8% and 79.9% identity, respectively). Amino acid sequence analysis of the primary structure of HtAATP revealed that it belonged to the plastidic ATP/ADP transporter family. Hydropathy prediction indicated that HtAATP gene product is a highly hydrophobic membrane protein that contains 10 transmembrane domains to form a spanning topology. Southern blotting analysis showed that the HtAATP gene is a single-copy gene in the H. tuberosus genome. Tissue distribution analysis showed that the HtAATP gene is prominently expressed in sink tissues. A stable expression pattern in tubers at different developmental stages implies an active involvement of HtAATP during carbohydrate formation.

Key words: expression pattern, Helianthus tuberosus, plastidic ATP/ADP transporter.

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