Nucleotide Variation in the NCED3 Region of Arabidopsis thaliana and its Association Study with Abscisic Acid Content under Drought Stress

doi:10.1111/j.1744-7909.2008.00786.x

Abstract

Abstract: Drought tolerance is a comprehensive quantitative trait being understood further at the molecular genetic level. Abscisic acid (ABA) is the main drought induced hormone which regulates the expression of many genes related to drought responses. Nine-cis-epoxycarotenoid dioxygenase (NCED3) is thought to be a key enzyme in ABA biosynthesis. In this paper, we measured the times of ABA content increase under drought stress, and sequenced and compared the sequence of AtNCED3 among 22 Arabidopsis thaliana accessions. The results showed that the times of ABA content increase under drought stress was highly variable among these accessions. High density of single nucleotide polymorphism (SNP) and insertion/deletion (Indel) were found in AtNCED3 region, on average 1 SNP per 87.4bp and 1 indel per 502 bp. Nucleotide diversity was significantly lower in coding region than that in non-coding region. The results of association study with ANOVA analysis suggested that the 274th site (PnS) and the 327th site (P nR) amino acid variations might be the cause of ABA content increase of 163av accession under drought stress.

Key words: Abscisic acid, Arabidopsis thaliana, association study, drought stress, nucleotide variation, NCED3 region.

Gang-Ping Hao, Xiu-Hai Zhang, Yong-Qin Wang, Zhong-Yi Wu and Cong-Lin Huang. Nucleotide Variation in the NCED3 Region of Arabidopsis thaliana and its Association Study with Abscisic Acid Content under Drought Stress[J]. J Integr Plant Biol., 2009, 51(2): 175-183.

[1]	Song Zhang, Kunyang Zhuang, Shiju Wang, Jinlian Lv, Na'na Ma, and Qingwei Meng. A novel tomato SUMO E3 ligase, SlSIZ1, confers drought tolerance in transgenic tobacco [J]. J Integr Plant Biol., 2017, 59(2): 102-117.
[2]	Sujittra Promchuea, Yujuan Zhu, Zhizhong Chen, Jing Zhang, and Zhizhong Gong. ARF2 coordinates with PLETHORAs and PINs to orchestrate ABA-mediated root meristem activity in Arabidopsis [J]. J Integr Plant Biol., 2017, 59(1): 30-43.
[3]	Zhongfei Li, Bin Li, Jian Liu, Zhihao Guo, Yuhao Liu, Yan Li, Wen-Hui Shen, Ying Huang, Hai Huang, Yijing Zhang, and Aiwu Dong. Transcription factors AS1 and AS2 interact with LHP1 to repress KNOX genes in Arabidopsis [J]. J Integr Plant Biol., 2016, 58(12): 959-970.
[4]	Arunkumar Srinivasan, José M. Jiménez-Gómez, Fabio Fornara, Wim J. J. Soppe, and Vittoria Brambilla. Alternative splicing enhances transcriptome complexity in desiccating seeds [J]. J Integr Plant Biol., 2016, 58(12): 947-958.
[5]	Bingxian Chen, Jun Ma, Zhenjiang Xu, and Xiaofeng Wang. Abscisic acid and ethephon regulation of cellulase in the endosperm cap and radicle during lettuce seed germination [J]. J Integr Plant Biol., 2016, 58(10): 859-869.
[6]	Shunhua Ding, Liang Wang, Zhipan Yang, Qingtao Lu, Xiaogang Wen, and Congming Lu. Decreased glutathione reductase2 leads to early leaf senescence in Arabidopsis [J]. J Integr Plant Biol., 2016, 58(1): 29-47.
[7]	Liang Yang, Qiaohong Liu, Zhibin Liu, Hao Yang, Jianmei Wang, Xufeng Li, and Yi Yang. Arabidopsis C3HC4-RING finger E3 ubiquitin ligase AtAIRP4 positively regulates stress-responsive abscisic acid signaling [J]. J Integr Plant Biol., 2016, 58(1): 67-80.
[8]	Haitao Shi, Tiantian Ye, Fan Yang, and Zhulong Chan. Arabidopsis PED2 positively modulates plant drought stress resistance [J]. J Integr Plant Biol., 2015, 57(9): 796-806.
[9]	Patrícia Vidigal, Ana Montserrat Martin-Hernandez, Cèlia Guiu-Aragonés, Sara Amâncio, and Luísa Carvalho. Selective silencing of 2Cys and type-IIB Peroxiredoxins discloses their roles in cell redox state and stress signaling [J]. J Integr Plant Biol., 2015, 57(6): 591-601.
[10]	Ji-Hee Min, Jung-Sung Chung, Kyeong-Hwan Lee, and Cheol Soo Kim. The CONSTANS-like 4 transcription factor, AtCOL4, positively regulates abiotic stress tolerance through an abscisic acid-dependent manner in Arabidopsis [J]. J Integr Plant Biol., 2015, 57(3): 313-324.
[11]	Martin Schäfer, Ivan D. Meza-Canales, Aura Navarro-Quezada, Christoph Brütting, Radomira Vanková, Ian T. Baldwin, and Stefan Meldau. Cytokinin levels and signaling respond to wounding and the perception of herbivore elicitors in Nicotiana attenuata [J]. J Integr Plant Biol., 2015, 57(2): 198-212.
[12]	Feng Wen, Tingting Qin, Yao Wang, Wen Dong, Aying Zhang, Mingpu Tan, and Mingyi Jiang. OsHK3 is a crucial regulator of abscisic acid signaling involved in antioxidant defense in rice [J]. J Integr Plant Biol., 2015, 57(2): 213-228.
[13]	Hao Du, Yu Chang, Fei Huang and Lizhong Xiong. GID1 modulates stomatal response and submergence tolerance involving abscisic acid and gibberellic acid signaling in rice [J]. J Integr Plant Biol., 2015, 57(11): 954-968.
[14]	Sarah Hatzig, L. Irina Zaharia, Suzanne Abrams, Marie Hohmann, Laurie Legoahec, Alain Bouchereau, Nathalie Nesi, and Rod J. Snowdon. Early osmotic adjustment responses in drought-resistant and drought-sensitive oilseed rape [J]. J Integr Plant Biol., 2014, 56(8): 797-809.
[15]	Ronny Brandt, Marc Cabedo, Yakun Xie, and Stephan Wenkel. Homeodomain leucine-zipper proteins and their role in synchronizing growth and development with the environment [J]. J Integr Plant Biol., 2014, 56(6): 518-526.

Nucleotide Variation in the NCED3 Region of Arabidopsis thaliana and its Association Study with Abscisic Acid Content under Drought Stress

HTML

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments