OsWR2 recruits HDA704 to regulate the deacetylation of H4K8ac in the promoter of OsABI5 in response to drought stress

doi:10.1111/jipb.13481

J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (7): 1651-1669.DOI: 10.1111/jipb.13481

• Abiotic Stress Responses • Previous Articles Next Articles

OsWR2 recruits HDA704 to regulate the deacetylation of H4K8ac in the promoter of OsABI5 in response to drought stress

Yalu Guo^1,2†, Yiqing Tan^3†, Minghao Qu^4†, Kai Hong³, Longjun Zeng⁵, Lei Wang⁶, Chuxiong Zhuang², Qian Qian⁷, Jiang Hu^7* and Guosheng Xiong^3*

1. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China;
2. Guangdong Laboratory for Lingnan Modern Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro‐bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;
3. Plant Phenomics Research Center, Academy of Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing 210095, China;
4. College of Animal Science and Technology, Southwest University, Chongqing 402460, China;
5. Yichun Academy of Sciences, Yinchun 336000, China;
6. Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, China;
7. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310000, China
^†These authors contributed equally to this work.
^*Correspondences: Guosheng Xiong (gsxiong@njau.edu.cn, Dr. Xiong is fully responsible for the distributions of the materials associated with this article); Jiang Hu (Hujiang588@163.com)

Received:2022-12-04 Accepted:2023-03-13 Online:2023-03-15 Published:2023-07-01

Abstract

Abstract: Drought stress is a major environmental factor that limits the growth, development, and yield of rice (Oryza sativa L.). Histone deacetylases (HDACs) are involved in the regulation of drought stress responses. HDA704 is an RPD3/HDA1 class HDAC that mediates the deacetylation of H4K8 (lysine 8 of histone H4) for drought tolerance in rice. In this study, we show that plants overexpressing HDA704 (HDA704-OE) are resistant to drought stress and sensitive to abscisic acid (ABA), whereas HDA704 knockout mutant (hda704) plants displayed decreased drought tolerance and ABA sensitivity. Transcriptome analysis revealed that HDA704 regulates the expression of ABA-related genes in response to drought stress. Moreover, HDA704 was recruited by a drought-resistant transcription factor, WAX SYNTHESIS REGULATORY 2 (OsWR2), and co-regulated the expression of the ABA biosynthesis genes NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3), NCED4, and NCED5 under drought stress. HDA704 also repressed the expression of ABA-INSENSITIVE 5 (OsABI5) and DWARF AND SMALL SEED 1 (OsDSS1) by regulating H4K8ac levels in the promoter regions in response to polyethylene glycol 6000 treatment. In agreement, the loss of OsABI5 function increased resistance to dehydration stress in rice. Our results demonstrate that HDA704 is a positive regulator of the drought stress response and offers avenues for improving drought resistance in rice.

Key words: drought stress, HDA704, histone acetylation, OsABI5, OsWR2, rice

Yalu Guo, Yiqing Tan, Minghao Qu, Kai Hong, Longjun Zeng, Lei Wang, Chuxiong Zhuang, Qian Qian, Jiang Hu and Guosheng Xiong. OsWR2 recruits HDA704 to regulate the deacetylation of H4K8ac in the promoter of OsABI5 in response to drought stress[J]. J Integr Plant Biol., 2023, 65(7): 1651-1669.

[1]	Zejun Hu, Fuan Niu, Peiwen Yan, Kai Wang, Lixia Zhang, Ying Yan, Yu Zhu, Shiqing Dong, Fuying Ma, Dengyong Lan, Siwen Liu, Xiaoyun Xin, Ying Wang, Jinshui Yang, Liming Cao, Shujun Wu and Xiaojin Luo. The kinase OsSK41/OsGSK5 negatively regulates amylose content in rice endosperm by affecting the interaction between OsEBP89 and OsBP5 [J]. J Integr Plant Biol., 2023, 65(7): 1782-1793.
[2]	Liang Zhou, Saihua Chen, Maohong Cai, Song Cui, Yulong Ren, Xinyue Zhang, Tianzhen Liu, Chunlei Zhou, Xin Jin, Limin Zhang, Minxi Wu, Shuyi Zhang, Zhijun Cheng, Xin Zhang, Cailin Lei, Qibing Lin, Xiuping Guo, Jie Wang, Zhichao Zhao, Ling Jiang, Shanshan Zhu and Jianmin Wan. ESCRT-III component OsSNF7.2 modulates leaf rolling by trafficking and endosomal degradation of auxin biosynthetic enzyme OsYUC8 in rice [J]. J Integr Plant Biol., 2023, 65(6): 1408-1422.
[3]	Kai Liu, Jijin Chen, Shang Sun, Xu Chen, Xinru Zhao, Yingying Hu, Guoxiao Qi, Xiya Li, Bo Xu, Jun Miao, Chao Xue, Yong Zhou and Zhiyun Gong. Histone deacetylase OsHDA706 increases salt tolerance via H4K5/K8 deacetylation of OsPP2C49 in rice [J]. J Integr Plant Biol., 2023, 65(6): 1394-1407.
[4]	Mengting Zhang, Yinyue Wang, Yuanyi Hu, Huacai Wang, Yawen Liu, Bingran Zhao, Jie Zhang, Rongxiang Fang and Yongsheng Yan. Heterosis in root microbiota inhibits growth of soil-borne fungal pathogens in hybrid rice [J]. J Integr Plant Biol., 2023, 65(4): 1059-1076.
[5]	Cong Sheng, Xuan Li, Shengge Xia, Yimai Zhang, Ze Yu, Cheng Tang, Le Xu, Zhaoyun Wang, Xin Zhang, Tong Zhou, Pingping Nie, Ayesha Baig, Dongdong Niu and Hongwei Zhao. An OsPRMT5-OsAGO2/miR1875-OsHXK1 module regulates rice immunity to blast disease [J]. J Integr Plant Biol., 2023, 65(4): 1077-1095.
[6]	Yifu Tian, Dating Zhong, Xinbo Li, Rundong Shen, Han Han, Yuqin Dai, Qi Yao, Xuening Zhang, Qi Deng, Xuesong Cao, Jian-Kang Zhu and Yuming Lu. High-throughput genome editing in rice with a virus-based surrogate system [J]. J Integr Plant Biol., 2023, 65(3): 646-655.
[7]	Yan Bi, Hui Wang, Xi Yuan, Yuqing Yan, Dayong Li and Fengming Song. The NAC transcription factor ONAC083 negatively regulates rice immunity against Magnaporthe oryzae by directly activating transcription of the RING-H2 gene OsRFPH2-6 [J]. J Integr Plant Biol., 2023, 65(3): 854-875.
[8]	Song Zhang, Kunyang Zhuang, Shiju Wang, Jinlian Lv, Na'na Ma, and Qingwei Meng. A novel tomato SUMO E3 ligase, SlSIZ1, confers drought tolerance in transgenic tobacco [J]. J Integr Plant Biol., 2017, 59(2): 102-117.
[9]	Xiaoba Wu, Jinxin Liu, Dongqi Li and Chun-Ming Liu. Rice caryopsis development I: Dynamic changes in different cell layers [J]. J Integr Plant Biol., 2016, 58(9): 772-785.
[10]	Xiaoba Wu, Jinxin Liu, Dongqi Li and Chun-Ming Liu. Rice caryopsis development II: Dynamic changes in the endosperm [J]. J Integr Plant Biol., 2016, 58(9): 786-798.
[11]	Huihui Liu, Chuanwei Yang and Lin Li. Shade-induced stem elongation in rice seedlings: Implication of tissue-specific phytohormone regulation [J]. J Integr Plant Biol., 2016, 58(7): 614-617.
[12]	Xiao-Ling Shang, Rong-Rong Xie, Hua Tian, Qing-Long Wang and Fang-Qing Guo. Putative zeatin O-glucosyltransferase OscZOG1 regulates root and shoot development and formation of agronomic traits in rice [J]. J Integr Plant Biol., 2016, 58(7): 627-641.
[13]	Deyong Ren, Yuchun Rao, Liwen Wu, Qiankun Xu, Zizhuang Li, Haiping Yu, Yu Zhang, Yujia Leng, Jiang Hu, Li Zhu, Zhenyu Gao, Guojun Dong, Guangheng Zhang, Longbiao Guo, Dali Zeng, and Qian Qian. The pleiotropic ABNORMAL FLOWER AND DWARF1 affects plant height, floral development and grain yield in rice [J]. J Integr Plant Biol., 2016, 58(6): 529-539.
[14]	Jinfeng Qi, Jiancai Li, Xiu Han, Ran Li, Jianqiang Wu, Haixin Yu, Lingfei Hu, Yutao Xiao, Jing Lu, and Yonggen Lou. Jasmonic acid carboxyl methyltransferase regulates development and herbivory-induced defense response in rice [J]. J Integr Plant Biol., 2016, 58(6): 564-576.
[15]	Xiao-Ming Zheng, Li Feng, Junrui Wang, Weihua Qiao, Lifang Zhang, Yunlian Cheng, and Qingwen Yang. Nonfunctional alleles of long-day suppressor genes independently regulate flowering time [J]. J Integr Plant Biol., 2016, 58(6): 540-548.

OsWR2 recruits HDA704 to regulate the deacetylation of H4K8ac in the promoter of OsABI5 in response to drought stress

HTML

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments