J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (5): 438-455.DOI: 10.1111/j.1744-7909.2009.00823.x

Special Issue: Sexual Reproductions

• Cell and Developmental Biology • Previous Articles     Next Articles

Comparative Proteomic Analysis of Arabidopsis Mature Pollen and Germinated Pollen

Junjie Zou1† Lianfen Song1† Wenzheng Zhang1, Yi Wang1, Songlin Ruan1,2 and Wei-Hua Wu1*   

  1. 1 State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
    2 Institute of Biotechnology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China
  • Received:2008-11-12 Accepted:2009-02-09 Published:2009-03-26
  • About author:These authors contributed equally to this work.
    *Author for correspondence Tel: +86 10 6273 1103; Fax: +86 10 6273 4640; E-mail: wuwh@public3.bta.net.cn

Abstract:

Proteomic analysis was applied to generating the map of Arabidopsis mature pollen proteins and analyzing the differentially expressed proteins that are potentially involved in the regulation of Arabidopsis pollen germination. By applying 2-D electrophoresis and silver staining, we resolved 499 and 494 protein spots from protein samples extracted from pollen grains and pollen tubes, respectively. Using the matrix-assisted laser desorption ionization time-of-flight mass spectrometry method, we identified 189 distinct proteins from 213 protein spots expressed in mature pollen or pollen tubes, and 75 new identified proteins that had not been reported before in research into the Arabidopsis pollen proteome. Comparative analysis revealed that 40 protein spots exhibit reproducible significant changes between mature pollen and pollen tubes. And 21 proteins from 17 downregulated and six upregulated protein spots were identified. Functional category analysis indicated that these differentially expressed proteins mainly involved in signaling, cellular structure, transport, defense/stress responses, transcription, metabolism, and energy production. The patterns of changes at protein level suggested the important roles for energy metabolism-related proteins in pollen tube growth, accompanied by the activation of the stress response pathway and modifications to the cell wall.
 

Zou J, Song L, Zhang W, Wang Y, Ruan S, Wu WH (2009). Comparative proteomic analysis of Arabidopsis mature pollen and germinated pollen. J. Integr. Plant Biol. 51(5), 438-455.

Key words: 2D-polyacrylamide gel electrophoresis, Arabidopsis thaliana, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, pollen, pollen tube, proteome.

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