J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (12): 1104-1115.DOI: 10.1111/j.1744-7909.2009.00879.x

Special Issue: Abiotic Stresses

• Plant-environmental Interactions • Previous Articles     Next Articles

Drought-stimulated Activity of Plasma Membrane NADPH Oxidase and its Catalytic Properties in Rice

Zhuang-Qin Duan 1,2, Lei Bai1,2, Zhi-Guang Zhao2, Guo-Ping Zhang 1, Fang-Min Cheng 1, Li-Xi Jiang1 and Kun-Ming Chen1*   

  1. 1Institute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China
    2 College of Life Sciences, Lanzhou University, Lanzhou 730000, China
  • Received:2008-12-19 Accepted:2009-08-31 Published:2009-11-26
  • About author: *Author for correspondence Tel: +86 571 8697 1905; Fax: +86 571 8697 1117; E-mail: kunmingchen@zju.edu.cn

Abstract:

The activity of plasma membrane (PM) nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and its catalytic properties in rice was investigated under drought stress conditions. Drought stress led to decreased leaf relative water content (RWC) and, as a result of drought-induced oxidative stress, the activities of antioxidant enzymes increased significantly. More interestingly, the intensity of applied water stress was correlated with increased production of H2O2 and O2 and elevated activity of PM NADPH oxidase, a key enzyme of reactive oxygen species generation in plants. Histochemical analyses also revealed increased H2O2 and O2 production in drought-stressed leaves. Application of diphenylene iodonium (DPI), an inhibitor of PM NADPH oxidase, did not alleviate drought-induced production of H2O2 and O2. Catalysis experiments indicated that the rice PM NADPH oxidase was partially flavin dependent. The pH and temperature optima for this enzyme were 9.8 and 40 ℃, respectively. In addition, drought stress enhanced the activity under alkaline pH and high temperature conditions. These results suggest that a complex regulatory mechanism, associated with the NADPH oxidase-H2O2 system, is involved in the response of rice to drought stress.
 

Duan ZQ, Bai L, Zhao ZG, Zhang GP, Cheng FM, Jiang LX, Chen KM (2009). Drought-stimulated activity of plasma membrane nicotinamide adenine dinucleotide phosphate oxidase and its catalytic properties in rice. J. Integr. Plant Biol. 51(12), 1104–1115.

Key words: catalytic properties, drought stress, plasma membrane NADPH oxidase, reactive oxygen species, rice (Oryza sativa).

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22