J Integr Plant Biol. ›› 2006, Vol. 48 ›› Issue (8): -.DOI: 10.1111/j.1744-7909.2006.00319.x

• Research Articles •    

Proteomic Analysis of the Response of Liangyoupeijiu (Super High-Yield Hybrid Rice) Seedlings to Cold Stress

Ping-Fang Yang, Xiao-Juan Li, Yu Liang, Yu-Xiang Jing, Shi-Hua Shen and Ting-Yun Kuang   

Abstract: Liangyoupeijiu is a super high-yield hybrid rice. Despite its advantages with respect to yield and grain quality, it is sensitive to cold, which keeps it from being widely cultivated. We subjected Liangyoupeijiu seedlings to 4 °C cold treatment, then extracted the leaf proteins. After 2-D gel electrophoresis separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis, a series of differentially displayed proteins were identified. Some metabolism-associated proteins were found among the downregulated proteins, such as carbamoyl phosphate synthetase, transketolase 1, dihydrolipoamide dehydrogenase and glyceraldehyde 3-phosphate dehydrogenase. The upregulated proteins included both stress-resistance proteins such as nucleoside diphosphate kinase I and proteins that are negative for rice growth, such as FtsH-like protein, plastid fusion and/or translocation factor (Pftf) and actin. Our results indicate that cold may inhibit Liangyoupeijiu growth through decreasing metabolic activity and damaging cell structure.(Author for correspondence.Tel:(0)10 6259 6545;Fax: (0)10 6259 6594E-mail:shshen@ibcas.ac.cn)

Key words: cold stress, Oryza sativa, proteome, super high-yield hybrid rice.

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