J Integr Plant Biol. ›› 2011, Vol. 53 ›› Issue (6): 480-492.DOI: 10.1111/j.1744-7909.2011.01050.x

• Research Articles • Previous Articles     Next Articles

A Gain-of-Function Mutation in IAA7/AXR2 Confers Late Flowering under Short-day Light in Arabidopsis

Yan-Xia Mai1, Long Wang1 and Hong-Quan Yang2*   

  1. 1National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
    2Plant Science Institute, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China
  • Received:2011-01-14 Accepted:2011-04-25 Published:2011-05-12
  • About author:*Corresponding author. Tel: 86-21-34206005; Fax: 86-21-34205877; Email: hqyang@sjtu.edu.cn

Abstract:

Floral initiation is a major step in the life cycle of plants, which is influenced by photoperiod, temperature, and phytohormones, such as gibberellins (GAs). It is known that GAs promote floral initiation under short-day light conditions (SDs) by regulating the floral meristem-identity gene LEAFY (LFY) and the flowering-time gene SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1). We have defined the role of the auxin signaling component INDOLE-3-ACETIC ACID 7 (IAA7)/AUXIN RESISTANT 2 (AXR2) in the regulation of flowering time in Arabidopsis thaliana. We demonstrate that the gain-of-function mutant of IAA7/AXR2, axr2-1, flowers late under SDs. The exogenous application of GAs rescued the late flowering phenotype of axr2-1 plants. The expression of the GA20 oxidase (GA20ox) genes, GA20ox1 and GA20ox2, was reduced in axr2-1 plants, and the levels of both LFY and SOC1 transcripts were reduced in axr2-1 mutants under SDs. Furthermore, the overexpression of SOC1 or LFY in axr2-1 mutants rescued the late flowering phenotype under SDs. Our results suggest that IAA7/AXR2 might act to inhibit the timing of floral transition under SDs, at least in part, by negatively regulating the expressions of the GA20ox1 and GA20ox2 genes.

Mai YX, Wang L, Yang HQ (2011) A gain-of-function mutation in IAA7/AXR2 confers late flowering under short-day light in Arabidopsis. J. Integr. Plant Biol. 53(6), 480–492.

Key words: Arabidopsis, GA 20-oxidases, GAs, IAA7/AXR2, auxin

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