J Integr Plant Biol. ›› 2012, Vol. 54 ›› Issue (2): 73-86.DOI: 10.1111/j.1744-7909.2012.01098.x

Special Issue: Plant Signal Transduction

• Plant-environmental Interactions • Previous Articles     Next Articles

Global Analysis of Non-coding Small RNAs in Arabidopsis in Response to Jasmonate Treatment by Deep Sequencing Technology

Bosen Zhang1,2, Zhiping Jin1,3* and Daoxin Xie1,2   

  1. 1School of Life Sciences, Tsinghua University, Beijing 100084, China
    2MOE Key Laboratory of Bioinformatics, Tsinghua University, Beijing 100084, China
    3State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China
  • Received:2011-08-14 Accepted:2011-12-22 Published:2012-01-06
  • About author: *Corresponding author. Tel: 010 6484 2382; Fax: 010 6487 3428; Email: jinzhiping@genetics.ac.cn

Abstract:

In plants, non-coding small RNAs play a vital role in plant development and stress responses. To explore the possible role of non-coding small RNAs in the regulation of the jasmonate (JA) pathway, we compared the non-coding small RNAs between the JA-deficient aos mutant and the JA-treated wild type Arabidopsis via high-throughput sequencing. Thirty new miRNAs and 27 new miRNA candidates were identified through bioinformatics approach. Forty-nine known miRNAs (belonging to 24 families), 15 new miRNAs and new miRNA candidates (belonging to 11 families) and 3 tasiRNA families were induced by JA, whereas 1 new miRNA, 1 tasiRNA family and 22 known miRNAs (belonging to 9 families) were repressed by JA.

Zhang B, Xie D, Jin Z (2012) Global analysis of non-coding small RNAs in Arabidopsis in response to jasmonate treatment by deep sequencing technology. J. Integr. Plant Biol. 54(2), 73–86.

Key words: Non-coding small RNAs, jasmonate, Arabidopsis, Solexa deep sequencing

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