J Integr Plant Biol. ›› 2013, Vol. 55 ›› Issue (5): 473-483.DOI: 10.1111/jipb.12021

Special Issue: Rice Genomics and Agriculture

• Original Article • Previous Articles     Next Articles

Characterization and Genetic Analysis of a Novel Rice Spotted-leaf Mutant HM47 with Broad-spectrum Resistance to Xanthomonas oryzae pv. oryzae

Bao-Hua Feng1†, Yang Yang1,2†, Yong-Feng Shi1, Hai-Chao Shen1, Hui-Mei Wang1, Qi-Na Huang1, Xia Xu1, Xiang-Guang Lü1 and Jian-Li Wu1*   

  1. 1State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
    2College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China
  • Received:2012-09-29 Accepted:2012-11-21 Published:2013-02-04
  • About author:These authors contributed equally to this work.
    *Corresponding author Tel: +86 571 6337 0326; Fax: +86 571 6337 0326; E-mail: beishangd@163.com

Abstract:

A stable inherited rice spotted-leaf mutant HM47 derived from an EMS-induced IR64 mutant bank was identified. The mutant expressed hypersensitive response (HR)-like symptoms throughout its whole life from the first leaf to the flag leaf, without pathogen invasion. Initiation of the lesions was induced by light under natural summer field conditions. Expression of pathogenesis-related genes including PAL, PO-C1, POX22.3 and PBZ1 was enhanced significantly in association with cell death and accumulation of H2O2 at and around the site of lesions in the mutant in contrast to that in the wild-type (WT). Disease reaction to Xanthomonas oryzae pv. oryzae from the Philippines and China showed that HM47 is a broad-spectrum disease-resistant mutant with enhanced resistance to multiple races of bacterial blight pathogens tested. An F2 progeny test showed that bacterial blight resistance to race HB-17 was co-segregated with the expression of lesions. Genetic analysis indicated that the spotted-leaf trait was controlled by a single recessive gene, tentatively named splHM47, flanked by two insertion/deletion markers in a region of approximately 74 kb on the long arm of chromosome 4. Ten open reading frames are predicted, and all of them are expressed proteins. Isolation and validation of the putative genes are currently underway.

Feng BH, Yang Y, Shi YF, Shen HC, Wang HM, Huang QN, Xu X, L¨uXG, Wu JL (2013) Characterization and genetic analysis of a novel rice spotted-leaf mutant HM47 with broad-spectrum resistance to Xanthomonas oryzae pv. oryzae. J. Integr. Plant Biol. 55(5), 473–483.

Key words: Rice, broad-spectrum disease resistance, physical map, pathogenesis-related genes, spotted-leaf

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