J Integr Plant Biol. ›› 2013, Vol. 55 ›› Issue (10): 902-916.DOI: 10.1111/jipb.12063

Special Issue: Cell Biology and Functions

• Cell and Developmental Biology • Previous Articles     Next Articles

Cotton AnnGh3 Encoding an Annexin Protein is Preferentially Expressed in Fibers and Promotes Initiation and Elongation of Leaf Trichomes in Transgenic Arabidopsis

Bing Li, Deng-Di Li, Jie Zhang, Hui Xia, Xiu-Lan Wang, Ying Li and Xue-Bao Li*   

  1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China
  • Received:2013-01-08 Accepted:2013-05-02 Published:2013-07-29
  • About author:These authors contributed equally to this paper.
    *Corresponding author: Tel/Fax: +86 27 6786 2443; E-mail: xbli@mail.ccnu.edu.cn

Abstract:

The annexins are a multifamily of calcium-regulated phospholipid-binding proteins. To investigate the roles of annexins in fiber development, four genes encoding putative annexin proteins were isolated from cotton (Gossypium hirsutum) and designated AnnGh3, AnnGh4, AnnGh5, and AnnGh6. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) results indicated that AnnGh3, AnnGh4, and AnnGh5 were preferentially expressed in fibers, while the transcripts of AnnGh6 were predominantly accumulated in roots. During fiber development, the transcripts of AnnGh3/4/5 genes were mainly accumulated in rapidly elongating fibers. With fiber cells further developed, their expression activity was dramatically declined to a relatively low level. In situ hybridization results indicated that AnnGh3 and AnnGh5 were expressed in initiating fiber cells (0–2 DPA). Additionally, their expression in fibers was also regulated by phytohormones and [Ca2+]. Subcellular localization analysis discovered that AnnGh3 protein was localized in the cytoplasm. Overexpression of AnnGh3 in Arabidopsis resulted in a significant increase in trichome density and length on leaves of the transgenic plants, suggesting that AnnGh3 may be involved in fiber cell initiation and elongation of cotton.

Li B, Li DD, Zhang J, Xia H, Wang XL, Li Y, Li XB (2013) Cotton AnnGh3 encoding an annexin protein is preferentially expressed in fibers and promotes initiation and elongation of leaf trichomes in transgenic Arabidopsis. J. Integr. Plant Biol. 55(10), 902–916.

Key words: Annexin, cotton (Gossypium hirsutum), fiber development, gene expression

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