J Integr Plant Biol. ›› 2015, Vol. 57 ›› Issue (2): 138-150.DOI: 10.1111/jipb.12218

• Molecular Physiology •     Next Articles

Arabidopsis transcriptional response to extracellular Ca2+ depletion involves a transient rise in cytosolic Ca2+

Jing Wang, Tergel Tergel, Jianhua Chen, Ju Yang, Yan Kang and Zhi Qi*   

  1. College of Life Sciences, Inner Mongolia University, Hohhot, China
  • Received:2014-03-04 Accepted:2014-05-19 Published:2014-05-21
  • About author:*Correspondence: E-mail: qizhi@imu.edu.cn

Abstract:

Ecological evidence indicates a worldwide trend of dramatically decreased soil Ca2+ levels caused by increased acid deposition and massive timber harvesting. Little is known about the genetic and cellular mechanism of plants' responses to Ca2+ depletion. In this study, transcriptional profiling analysis helped identify multiple extracellular Ca2+ ([Ca2+]ext) depletion-responsive genes in Arabidopsis thaliana L., many of which are involved in response to other environmental stresses. Interestingly, a group of genes encoding putative cytosolic Ca2+ ([Ca2+]cyt) sensors were significantly upregulated, implying that [Ca2+]cyt has a role in sensing [Ca2+]ext depletion. Consistent with this observation, [Ca2+]ext depletion stimulated a transient rise in [Ca2+]cyt that was negatively influenced by [K+]ext, suggesting the involvement of a membrane potential-sensitive component. The [Ca2+]cyt response to [Ca2+]ext depletion was significantly desensitized after the initial treatment, which is typical of a receptor-mediated signaling event. The response was insensitive to an animal Ca2+ sensor antagonist, but was suppressed by neomycin, an inhibitor of phospholipase C. Gd3+, an inhibitor of Ca2+ channels, suppressed the [Ca2+]ext-triggered rise in [Ca2+]cyt and downstream changes in gene expression. Taken together, this study demonstrates that [Ca2+]cyt plays an important role in the putative receptor-mediated cellular and transcriptional response to [Ca2+]ext depletion of plant cells.

 

Wang J, Tergel T, Chen J, Yang J, Kang Y, Qi Z (2015) Arabidopsis transcriptional response to extracellular Ca2+ depletion involves a transient rise in cytosolic Ca2+. J Integr Plant Biol 57: 138–150. doi: 10.1111/jipb.12218

Key words: Arabidopsis, aequorin, calcium depletion, cytosolic calcium, transcriptional response

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