J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (1): 102-104.

• Research Articles • Previous Articles     Next Articles

Preliminary Analysis of Cytoplasmic Genome of Diploid Somatic Hybrid Derived from Fusion Between Rough Lemon and Hamlin Sweet Orange

LIU Ji-Hong, DENG Xiu-Xin   


RFLP (restriction fragment length polymorphism) was employed to analyze cytoplasmic genome of diploid somatic hybrid plant, morphologically similar to rough lemon which was leaf parent, that was produced via protoplast fusion between rough lemon (Citrus jambhiri Lush) and Hamlin sweet orange (C. sinensis Osb.), the embryogenic parent. Three enzyme-mitocondrial probe combinations and one enzyme-chloroplast probe combination demonstrated that the plant had identical band profiles to Hamlin sweet orange as far as mtDNA and cpDNA were concerned.

刘继红 邓秀新*

关键词: 柑橘;胞质杂种;RFLP;线粒体基因组

Key words: citrus, hybrid, RFLP, mitocondrial genome

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