J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (11): 1101-1107.

• Research Articles •     Next Articles

Progresses and Applications of Fluorescence in Situ Hybridization

WANG Ling, NING Shun-Bin, SONG Yun-Chun, L Ying-Tang   


The techniques of in situ hybridization (ISH) are widely adopted for analyzing the genetic make-up and RNA expression patterns of individual cells. There are four main criterions for evaluating this technique, including detection sensitivity, resolution, capacity and specificity. This review focuses on a number of advances made over the last years in the fluorescence in situ hybridization (FISH). These advances can be catagorized into several branches as follows: (1) Multicolor-FISH (mFISH), including conventional mFISH, combinatorial FISH, ratio labelling FISH, multicolor chromosome painting and comparative genomic hybridization (CGH); (2) Extended DNA fiber-FISH (EDF-FISH), including quantitative DNA fiber mapping (QDFM), molecular combing (MC) and dynamic molecular combing (DMC); (3)In situ PCR-based FISH; (4) Bacterial (or yeast) artificial chromosome-FISH (BAC-FISH or YAC-FISH); (5) Tyramide signal amplification-FISH (TSA-FISH); (6) Polypeptide nucleic acid-FISH (PNA-FISH) and (7) padlock-FISH.

王玲 宁顺斌 宋运淳 吕应堂*

关键词: 荧光原位杂交;多色荧光原位杂交;DNA 纤维;分辨率;灵敏度;特异性;物理作图;RNA表达图谱

Key words: FISH, multicolor-FISH, DNA fiber, resolution, sensitivity, specificity, physical mapping, RNA expression pattern

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