J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (11): 1101-1107.
• Research Articles • Next Articles
WANG Ling, NING Shun-Bin, SONG Yun-Chun, L Ying-Tang
Abstract:
The techniques of in situ hybridization (ISH) are widely adopted for analyzing the genetic make-up and RNA expression patterns of individual cells. There are four main criterions for evaluating this technique, including detection sensitivity, resolution, capacity and specificity. This review focuses on a number of advances made over the last years in the fluorescence in situ hybridization (FISH). These advances can be catagorized into several branches as follows: (1) Multicolor-FISH (mFISH), including conventional mFISH, combinatorial FISH, ratio labelling FISH, multicolor chromosome painting and comparative genomic hybridization (CGH); (2) Extended DNA fiber-FISH (EDF-FISH), including quantitative DNA fiber mapping (QDFM), molecular combing (MC) and dynamic molecular combing (DMC); (3)In situ PCR-based FISH; (4) Bacterial (or yeast) artificial chromosome-FISH (BAC-FISH or YAC-FISH); (5) Tyramide signal amplification-FISH (TSA-FISH); (6) Polypeptide nucleic acid-FISH (PNA-FISH) and (7) padlock-FISH.
荧光原位杂交技术的发展与应用 王玲 宁顺斌 宋运淳 吕应堂*
(武汉大学植物发育生物学教育部重点实验室,武汉430072) 关键词: 荧光原位杂交;多色荧光原位杂交;DNA 纤维;分辨率;灵敏度;特异性;物理作图;RNA表达图谱
Key words: FISH, multicolor-FISH, DNA fiber, resolution, sensitivity, specificity, physical mapping, RNA expression pattern
WANG Ling, NING Shun-Bin, SONG Yun-Chun, L Ying-Tang. Progresses and Applications of Fluorescence in Situ Hybridization[J]. J Integr Plant Biol., 2000, 42(11): 1101-1107.
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