Abstract:

R5002-12, a salt tolerant line of Phragmites communis Trin., which was obtained from ethyl methane sulfonate (EMS) treated callus selected under saline stress, was compared with its wild line in respect to their molecular biological, physiological and biochemical characterizations. Five arbitrary primers were screened which showed differences in DNA amplified polymorphism between the variant and its wild line. Some new proteins appeared in the salt tolerant plant under salt stress. Electrophoresis of peroxidase and esterase also showed some differences in isozyme expression between them. The chlorophyll content of the variant was higher than that of the original variety, whether the plants were under salt stress or not.

芦苇耐盐变异体与野生型植株某些特性的比较
许祥明 叶和春* 李国凤 陈建林
（中国科学院植物研究所，北京100093）

摘要：对通过细胞工程方法得到的耐盐芦苇（Ｐｈｒａｇｍｉｔｅｓ ｃｏｍｍｕｎｉｓ Ｔｒｉｎ．）变异系Ｒ５００２－１２与野生型植株进行了分子生物学和生理生化特性比较分析。筛选到５个ＲＡＰＤ引物对变异体和野生型植株的ＤＮＡＦ的随机扩增表现出不同的多态性，表明该变异体在分子水平上发生了变化。生理生化分析的结果表明，平行生长状态下的两种株系，其可溶性蛋白和同工酶的表达水平和种类不一样，变异体在ＮａＣｌ胁迫下，表达特异性的蛋白。实验还表明，无论胁迫
与否，变异体的叶绿素含量都高于野生型植株，生长量比野生型植株大。
关键词： 芦苇；耐盐变异体；RAPD；同工酶；叶绿素；可溶性蛋白

Key words: Phragmites communis, salt tolerant variant, RAPD, isozyme, chlorophyll, soluble protein