Abstract:

In rice (Oryza sativa L.) roots two glutamine synthetase (GS) isozymes, GSra and GSrb, were identified recently in the author's experiments, but the homology of both GSra and GSrb as well as their localization in the rice roots are unclear. In the present study, the purified GSra and GSrb from rice roots were used to immunize rabbits to obtain the respective antibodies. The immunodiffusion and immunoblotting experiments showed that the antibody against GSra or GSrb was specific for GS and its isozymes. The immunoprecipitation test indicated that the antibody of GSra or GSrb not only recognized its respective antigen, but also well recognized each other's antigen. GSra or GSrb antibody recognized also better cytosolic GS1 of rice leaves, but the recognization for chloroplast GS2 from rice or spinach (Spinacia oleracea Mill.) leaves was weaker. Our results indicate that GSra and GSrb from rice roots are quite similar in antigenicity and are extremely similar proteins and that both GSra and GSrb may also be a form of cytosolic GS just as the cytosolic GS1 of rice leaves.

水稻谷氨酰胺合成酶同工酶免疫学性质比较研究
林清华¹ 李常健¹ 张楚富^1* 彭进¹ PENG Shao-Bing² John BENNETT ²

（1.  武汉大学生命科学学院，武汉430072；/2.International Rice Research Institute,P.O. Box 3127,1271 Makati City,Philippines）

Key words: antigen, antibody, glutamine synthetase, isozyme, rice