J Integr Plant Biol. ›› 2001, Vol. 43 ›› Issue (5): 469-474.

• Research Articles • Previous Articles     Next Articles

Molecular Cytogenetic Analysis of Winter Wheat Germplasm Aimengniu

QI Zeng-Jun, LIU Da-Jun, CHEN Pei-Du, LI Qing-Qi   

Abstract:

Chromosome constitution of the major types winter wheat ( Triticum aestivum L.) germplasm, Aimengniu derived from Aifeng 3//Mengxian 201/Neuzucht, was examined through mitotic chromosome C-banding and fluorescence in situ hybridization (FISH). The results indicated that Aimengniu Ⅱ, Ⅳ, Ⅴ and Ⅵ involved a new wheat-rye complex translocation, which was probably resulted from a second reciprocal translocation between the first translocation chromosome 1RS·1BL and wheat chromosome 7D. It was designated as 1RS·7DS and 1BL·7DL. The results also revealed that Aimengniu Ⅶ contained a usual 1RS·1BL and no translocation between wheat and rye was found in Aimengniu Ⅲ.

冬小麦种质“矮孟牛”的分子细胞遗传学研究
亓增军1  刘大钧1* 陈佩度1  李晴祺2

(1. 南京农业大学农业部作物细胞遗传重点开放实验室,南京210095;2.  山东农业大学农学系,泰安271018)

摘要:利用染色体C分带和FISH(fluorescence in situ hybridization)技术对冬小麦(Triticumaestivum L.)种质“矮孟牛"Ⅱ、Ⅳ、Ⅴ型及其在选育过程中产生而未被生产上利用的Ⅲ、Ⅵ、Ⅶ型进行了鉴定与分析。结果表明,被鉴定的“矮孟牛"6种类型染色体数均为2n=42,其中,“矮孟牛"Ⅶ型为1RS.1BL易位,“矮孟牛"Ⅲ型染色体组成中未发现小麦-黑麦易位,“矮孟牛”Ⅱ、Ⅳ、Ⅴ、Ⅵ型则存在一种新的小麦-黑麦复杂易位,亦即在杂交过程中产生的小麦-黑麦1RS.1BL易位染色体又与另一条小麦染色体7D发生了相互易位,将其确定为1RS.7DS和1BL.7DL。

关键词: 小麦;黑麦;复杂易位;1RS.1BL 分带: fluorescence in situ hybridization;冬小麦种质“矮孟牛”

Key words: wheat, rye, complex translocation, 1RS·1BL, C-banding, fluorescence in situ hybridization, winter wheat germplasm Aimengniu

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