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J Integr Plant Biol, 2001, 43 (7): 704-708, Research Article
Kinetic Fluorescence Spectral Analysis of Core Antennas CP43 and CP47 of Photosystem Ⅱ with Ultrafast Time-resolved Technology
HE Jun-Fang, WANG Shui-Cai, ZHANG Shu, HE Feng-Tao, SHAN Ji-Xiu, KUANG Ting-Yun
doi:
Abstract

Ultrafast time-resolved fluorescence experiments have been performed with core antennas CP43 and CP47 of PS Ⅱ. Their dynamic fluorescence spectra were obtained with excitation wavelength 514.5 nm. For CP43, the emission spectrum was found to be from 640 to 780 nm with a peak at ~680 nm and the lifetime of fluorescence was 3.54 ns. For CP47, the emission spectrum was from 630 to 775 nm with a peak at ~691 nm and the fluorescence lifetime was 3.22 ns. The fluorescence emission efficiencies of Chl a in CP43 and CP47 were calculated to be approximately 38.3% and 40.6%, respectively. The energy transfer from β-Car to Chl a in CP43 and CP47 was discussed. The rates of energy transfer from β-Car to Chl a were measured to be about 9.6×1011 s-1 and 1.3×1012 s-1 and energy transfer efficiencies 47.5% and 66.5% respectively. The edge-edge distances between β-Car and Chl a in CP43 and CP47 were estimated to be ~0.110 nm and ~0.085nm respectively.

利用超快时间分辨技术对光系统II核心天线CP43和CP47的动力学荧光光谱的分析
贺俊芳1,2王水才1 张舒1,2 贺锋涛1,2 单际修3 匡廷云3

(1.  中国科学院西安光学精密机械研究所瞬态光学技术国家重点实验室,西安710068;2."$ 西北大学现代物理研究所,西安710069);3. 中国科学院植物研究所光合作用基础研究开放实验室,北京100093)

摘要:采用超快时间分辨荧光光谱装置对光系统Ⅱ核心天线CP43和CP47进行了研究 ,并在 5 14.5nm激光激发下获得了它们的动力学荧光光谱。CP43和CP47的荧光光谱范围分别为 6 40~ 780nm和 6 30~ 775nm ,并且它们分别在约 6 80nm和 6 91nm处有最大峰 ,在这两个峰值处的荧光寿命分别约为 3.5 4ns和 3.2 2ns。通过理论计算认为在CP43和CP47中 ,叶绿素a的荧光发射效率分别约为 38.3%和 40 .6 %。讨论了类胡萝卜素到叶绿素a分子的能量传递 ,认为在CP43和CP47中 ,类胡萝卜素到叶绿素a分子的能量传递时间常数分别为 9.6× 10 11s-1和 1.3× 10 12s-1,能量传递效率分别为 47.5 %和 6 6 .5 % ,并且估计在这两种核心天线中 ,类胡萝卜素分子和叶绿素a分子的外周间距分别约为 0 .110nm和 0 .0 85nm。
 

关键词: 核心天线;CP43;CP47;类胡萝卜素;叶绿素a;能量传递;光系统II

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