J Integr Plant Biol. ›› 2002, Vol. 44 ›› Issue (10): 1203-1208.

• Research Articles • Previous Articles     Next Articles

Cloning and Characterization of the Na+/H+ Antiport Genes from Triticum aestivum

WANG Zi Ning, ZHANG Jin Song, GUO Bei Hai, HE Si Jie, TIAN Ai Guo, CHEN Shou Yi   

Abstract:

The Na+/H+ antiport genes named TaNHX1 and TaNHX2 were cloned by screening a salt stressed wheat cDNA library using rice Na+/H+ antiport cDNA fragment as the probe. Sequencing analysis showed that TaNHX1 was 2 029 bp in length and contained a complete ORF of 1 638 bp. The TaNHX1 encodes a polypeptide of 546 amino acids with a transmembrane domain DIFFIYLLPPI. TaNHX2 was 1 693 bp in length consisting of a partial ORF followed by a 3′ UTR of 808 bp. The amino acid sequence of these two genes were about 70% identical to the known NHX genes from rice, Arabidopsis and Atriplex. ART PCR assay showed that the level of TaNHX1 transcripts was increased and reached a steady higher level in the seedlings after 3 h treatment with 400 mmol/L NaCl.

小麦Na+/h+反转运蛋白基因的克隆和特性
王子宁1,2 张劲松1 郭北海2 何锶洁1 田爱国1 陈受宜1*

(1. 中国科学院遗传研究所植物生物技术重点实验室,北京100101;2. 河北农林科学院粮油作物研究所作物育种室,石家庄050031)

摘要: 以水稻(Oryza sativa L.) Na+/H+反转运蛋白cDNA片段为探针,从小麦盐胁迫cDNA文库中筛选和克隆了2个小麦Na+/H+反转运蛋白基因,分别命名为TaNHX1 TaNHX2.序列分析表明TaNHX1为2 029 bp,包含一个完整的1 638 bp的ORF,编码546个氨基酸,其中含有DIFFIYLLPPI跨膜区.TaNHX2为1 693 bp,包含部分ORF及808 bp的3′-UTR.这2个基因与已知的水稻、拟南芥(Arabidopsis thialiana)和滨藜(Atriplex gmelini)中的同类基因NHX的相似性约为70%.RT-PCR分析表明小麦苗经400 mmol/L NaCl处理1 h后,TaNHX1的转录水平有所提高.

关键词: 小麦;Na+/H+ 反转运蛋白基因;盐诱导

Key words: wheat, Na+/H+ antiport gene, salt inducible

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