J Integr Plant Biol. ›› 2003, Vol. 45 ›› Issue (12): 1465-1474.

• Research Articles • Previous Articles     Next Articles

Programmed Cell Death During Secondary Xylem Differentiation in Eucommia ulmoides

CAO Jing, HE Xin-Qiang, WANG Ya-Qing, Sodmergen, CUI Ke-Ming   


Programmed cell death (PCD) during secondary xylem differentiation in Eucommia ulmoides Oliv. was examined using electron microscopy and by investigation of DNA fragmentation and degradation of caspase-like proteases (CLPs). DNA ladders were detected in developing secondary xylem by gel electrophoresis. DNA fragmentation was further confirmed by using the TdT-mediated dUTP nick-end labeling (TUNEL) method. Western blotting analysis showed that CLPs (caspase-8- and caspase-3-like proteases) and PARP (poly (ADP-ribose) polymerase) were degraded during secondary xylem differentiation. The results thus indicated that secondary xylem differentiation in E. ulmoides was a typical process of PCD and the degradation of CLPs might be a constitutive PCD event during secondary xylem differentiation.


Key words: caspase-like protease, DNA fragmentation, Eucommia ulmoides , poly (ADP-ribose) polymerase, programmed cell death, secondary xylem differentiation

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