J Integr Plant Biol. ›› 2003, Vol. 45 ›› Issue (12): 1475-1480.

• Research Articles • Previous Articles     Next Articles

Establishment of In Vitro Regeneration System of the Atrichum Mosses

GANG Yong-Yun, DU Gui-Sen, SHI Ding-Ji , WANG Mei-Zhi , LI Xue-Dong , HUA Zhen-Ling   


In vitro regeneration systems of Atrichum mosses, Atrichum undulatum (Hedw.) P. Beauv. and A. undulatum var. minus (Hedw.) Par. were established. After one month, soft, friable and green calli were induced successfully from inoculated protonema of Atrichum mosses on MS medium containing glucose (4%) and 6-BA (0.2-2.0 mg/L). The suitable culture medium for the callus induction and regular subculture was MS medium with 1.0-2.0 mg/L 6-BA and 4% glucose. The calli of Atrichum mosses developed into protonema, when it was transferred to phytohormone-free MS medium with 4% glucose. Meanwhile, the calli developed into erect gametophytes through protonema phase on carbohydrate-free Benecke medium.


Key words: mosses, protonema, callus, redifferentiation

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