J Integr Plant Biol. ›› 2004, Vol. 46 ›› Issue (7): 854-861.

• Research Articles • Previous Articles     Next Articles

Production and Analysis of Transgenic Maize with Improved Salt Tolerance by the Introduction of AtNHX1 Gene

YIN Xiao-Yan, YANG Ai-Fang, ZHANG Ke-Wei, ZHANG Ju-Ren   

Abstract:

The AtNHX1 gene and hygromycin phosphotransferase (hpt) gene were introduced into the embryonic callus cells of maize (Zea mays L.) elite inbred lines DH4866, Qi 319 and Luyuan 16106 by Agrobacterium-mediated method. Plantlets were obtained from the hygromycin-resistant calli after screening. PCR analysis and Southern blot hybridization indicated that there were 22.8% transgenic plants among the regenerated plants. The transformation frequencies were obviously affected by Agrobacterium concentration, the genotype of callus and co-cultivation duration. The segregations of AtNHX1 gene in the progeny were not easily to be elucidated, and some lines showed Mendelian segregations. The screening of salt tolerance indicated that the salt tolerance of some transgenic plants and their progenies were improved significantly and the seeds of some lines were able to germinate and grow in the presence of 0.8%-1.0% sodium chloride. Northern blotting analysis of T2 transgenic plants indicated that the salt tolerance of plants was correlative with the transcription level of AtNHX1 in the leaves.

Key words: maize, AtNHX1 gene, Agrobacterium , transgenic plant, salt tolerance

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22