J Integr Plant Biol. ›› 2004, Vol. 46 ›› Issue (8): 982-987.

• Research Articles • Previous Articles     Next Articles

Expression of the Pathogenesis-related Gene Promoter—GUS Reporter Fusion in Arabidopsis

ZHAO Shu-Qing, LI Xin-Feng, GUO Jian-Bo   


Pathogesis-related gene 1 (PR1) is a valid marker gene for systemic acquired resistance in Arabidopsis thaliana L. Using PCR amplification, we cloned the upstream regulatory region of PR1 gene from Arabidopsis Col-0. The fragment was then fused to the reporter gene encoding b-glucuronidase (GUS) and introduced into Arabidopsis plant by Agrobacterium-mediated gene transfer. PCR analysis and Southern blot hybridization of total DNA extracted from transgenic plants verified that the fusion gene had been integrated into the Arabidopsis genome. GUS activity can be induced by chemical treatment in transgenic Arabidopsis. The response was monitored by histochemical staining of GUS activity in situ, and fluorimetric assay in tissue extracts. Transgenic plants containing PR1-GUS or other defense gene promoter-reporter gene fusions may therefore provide specific assay systems for screening potential activators of systemic acquired resistance.

Key words: Arabidopsis thaliana , pathogenesis-related gene promoter, reporter gene, transformation

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q