Drought-stimulated Activity of Plasma Membrane NADPH Oxidase and its Catalytic Properties in Rice

doi:10.1111/j.1744-7909.2009.00879.x

J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (12): 1104-1115.DOI: 10.1111/j.1744-7909.2009.00879.x

• Plant-environmental Interactions • Previous Articles Next Articles

Drought-stimulated Activity of Plasma Membrane NADPH Oxidase and its Catalytic Properties in Rice

Zhuang-Qin Duan ^1,2, Lei Bai^1,2, Zhi-Guang Zhao², Guo-Ping Zhang ¹, Fang-Min Cheng ¹, Li-Xi Jiang¹ and Kun-Ming Chen^1*

¹Institute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China
² College of Life Sciences, Lanzhou University, Lanzhou 730000, China

Received:2008-12-19 Accepted:2009-08-31 Published:2009-11-26
About author: ^*Author for correspondence Tel: +86 571 8697 1905; Fax: +86 571 8697 1117; E-mail: kunmingchen@zju.edu.cn

Abstract

Abstract:

The activity of plasma membrane (PM) nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and its catalytic properties in rice was investigated under drought stress conditions. Drought stress led to decreased leaf relative water content (RWC) and, as a result of drought-induced oxidative stress, the activities of antioxidant enzymes increased significantly. More interestingly, the intensity of applied water stress was correlated with increased production of H₂O₂ and O₂⁻ and elevated activity of PM NADPH oxidase, a key enzyme of reactive oxygen species generation in plants. Histochemical analyses also revealed increased H₂O₂ and O₂⁻ production in drought-stressed leaves. Application of diphenylene iodonium (DPI), an inhibitor of PM NADPH oxidase, did not alleviate drought-induced production of H₂O₂ and O₂⁻. Catalysis experiments indicated that the rice PM NADPH oxidase was partially flavin dependent. The pH and temperature optima for this enzyme were 9.8 and 40 ℃, respectively. In addition, drought stress enhanced the activity under alkaline pH and high temperature conditions. These results suggest that a complex regulatory mechanism, associated with the NADPH oxidase-H₂O₂ system, is involved in the response of rice to drought stress.

Duan ZQ, Bai L, Zhao ZG, Zhang GP, Cheng FM, Jiang LX, Chen KM (2009). Drought-stimulated activity of plasma membrane nicotinamide adenine dinucleotide phosphate oxidase and its catalytic properties in rice. J. Integr. Plant Biol. 51(12), 1104–1115.

Key words: catalytic properties, drought stress, plasma membrane NADPH oxidase, reactive oxygen species, rice (Oryza sativa).

Zhuang-Qin Duan , Lei Bai, Zhi-Guang Zhao, Guo-Ping Zhang , Fang-Min Cheng , Li-Xi Jiang, and Kun-Ming Chen. Drought-stimulated Activity of Plasma Membrane NADPH Oxidase and its Catalytic Properties in Rice[J]. J Integr Plant Biol., 2009, 51(12): 1104-1115.

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Drought-stimulated Activity of Plasma Membrane NADPH Oxidase and its Catalytic Properties in Rice

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