J Integr Plant Biol. ›› 1991, Vol. 33 ›› Issue (12): -.

• Research Articles •    

The Construction of the Plasmid Containing the Chimaeric Patatin-GUS Gene and Its Expression in Potato

Ming Zhen-huan and G. Ooms   

Abstract: A 3.8 kb fragment containing 5’ flanking DNA of the patatin gene was used to construct a transcriptional fusion gene with the coding DNA of the bacterial glucuronidase (GUS) gene and the termination sequence of the nopaline synthase gene (nos). Plasmid pPOT414 containing the chimaeric gene construct was transferred from E. coli strain JM83 to A. grobacterium tumefaciens strain LBA4404 (pRAL4404) by the triparental mating method. Then, the chimaeric gene was introduced into potato cultivar Desiree by agrobacterial transformation of tissue slices'. Regenerated plants were selected by growth on media containing kanamycin. GUS activity was measured in extracts of leaf, stem, root and tuber tissues from independent transgenic lines and chimaeric GUS gene was expressed in all transgenic plants. To the same transgenic lines, GUS activities in light cultured shoots were significantly higher than those in dark cultured ones. It seems that the expression of the chimaeric gene was probably regulated by light.

Key words: Patatin, β-Glucuronidase (GUS), Chimaeric gene, Transgenic plant, Gene expression

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