J Integr Plant Biol. ›› 1991, Vol. 33 ›› Issue (6): -.

• Research Articles •    

Identification of Alien Chromatin and Ribosomal DNA in Wheat by in Situ Hybridization

Zhong Shao-bin, Zhang De-yu, Zhou Nan, Jiang Jian-dong, Yao Jing-xia, A. R. Leitch and I. J. Leitch   

Abstract: In situ hybridization was carried out to somatic cells of hexaploid Triticale “Badger”, lB/IR translocation line “Ning 8026” and IR(ID) substitution line “84056-1-36-1” using biotin-labelled total rye genomic DNA and wheat rDNA as probes, the results were as follows: 1. The probe containing the total genomic DNA from rye hybridized to the entire length of all rye chromosomes, as a result of the formation of a brown precipitate over the sites of hybridization, the rye chromosomes could be distinguished from wheat chromosomes counterstained by Wright’s solution, the distinguishable appearance of the wheat and rye chromosomes resulted in an efficient method of detecting rye chromosome or segments in wheat. 2. When the probe PTA 71 containing wheat ribosomal DNA was used to hybridize to somatic chromosomes of "Badger" and “84056-1-36-1”, six signals in “Badger” and eight in “84056-1-36-1” were observed on lB, 6B, 1R and SD, among which lB and 6B showed large in situ signals corresponding to many copies of the genes. 3. The expression behavior of wheat rDNA was found in interphase cells by in situ hybridization.

Key words: Triticum aestivum, Secale cereal, Triticale, In situ hybridization, Genomic probes, rDNA, PTA 71

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