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J Integr Plant Biol, 1992, 34 (7): -, Research Article
Amplification of Soybean Mosaic Virus Coat Protein Gene by Polymerase Chain Reaction and Its Sequence Analysis
Chu Rui-yin, Leng Xiao-hong, Bao Yi-ming, Pu Zu-qin,Pan Nai-sui and Chen Zhang-liang
doi:
Abstract
The results of cloning and sequencing of the gene encoding coat protein (CP) of soybean mosaic virus with PCR technique are reported. The virus RNA was extracted from purified viral particles. The first strand of cDNA was synthesized from viral RNA template primed with synthetic 3′polymerase chain reaction (PCR) primer using AMV reverse transcriptase. A DNA fragment with 0.8 kb was obtained after 30 PCR amplification cycles. The restriction map of the DNA fragment has been analyzed and its whole DNA sequence has been determined. The results show that the entire gene encoding the coat protein of soybean mosaic virus (SMV) has been cloned. The homologies of the DNA sequences and the deduced amino acid sequence between the SMV Sa strain shown here and the N strain of SMV published abroad are 96% and 96.6% respectively. The work of tranferring the CP gene into soybean to obtain transgenic soybean plants that resist to SMV infection is in progress.
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