J Integr Plant Biol. ›› 1996, Vol. 38 ›› Issue (11): -.

• Research Articles •    

Genetic Mapping of Rice Using RFLP Markers and a Double Haploid Population of a Cross Between indica and japonica Varieties

Li Ping, Zhu Li-huang, Zhou Kai-da, Chen Ying, Lu Chao-fu and He ping   

Abstract: A genetic linkage map of rice was constructed using a double haploid (DH) population from "Gui 630” (Oryza sativa subsp, indica)/"02428" (O. sativa subsp, japonica, wide compatibility variety) and RFLP markers. It consists of 233 loci and covers rice genomes about 2070 cM (centimorgan), and compares well with the other published rice maps. 25 RFLP markers, 2 telomeres and sh-2 (shattering ability) gene were first located on the molecular map of rice. RFLPs between "Gui 630' and "02428' mainly came from base substitution and a few DNA construction variance, not distributed evenly among chromosomes and on chromosome. This was probably resulted from the difference genetic stability among chromosomes and regions, in exchanging recombination ability in different segments of chromosome.

Key words: Rice, Ristriction fragment lenth polymorphisim, Genetic mapping, Double Haploid

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