Hybridization of Aegilops caudata with Triticum durum, Triticum aestivum and  the Detection of Alien Chromatins

Abstract

Abstract: Aegilops caudata L. carries resistance genes against wheat diseases as well as genes of high crude protein and lysine contents, which can be useful for wheat improvement. An amphiploid of Triticum durum - Ac. caudata was synthesized and the hybridization of T. aestivum with the amphiploid Am 8 was carried out. Chromosome in situ hybridization was carried out for the PMC (pollen mother cell) of the synthesized amphiploid (AABBCC) and ( T. aestivum Beijing 837 × Am 8) F2 by using the pAeca 212 as a probe. The results showed that the 7 bivalents from C genome had hybridization signals in the amphiploid. The detection for F2(Beijing 837× Am 8) indicated that translocation, even (pure) home translocation, occurred in F2 generations spontaneously. The study showed the bright prospect in transferring alien resistance genes from C genome to wheat.

Key words: Triticum aestivum, Aegilops caudata, Amphiploid, In situ hybridization

KONG Xiu-Ying, ZHOU Rong-Hua, DONG Yu-Shen and JIA Ji-Zeng. Hybridization of Aegilops caudata with Triticum durum, Triticum aestivum and the Detection of Alien Chromatins[J]. J Integr Plant Biol., 1999, 41(11): -.

[1]	Jianhui Ma, Yuke Geng, Hong Liu, Mengqi Zhang, Shujuan Liu, Chenyang Hao, Jian Hou, Youfu Zhang, Daijing Zhang, Weijun Zhang, Xueyong Zhang and Tian Li. TaTIP41 and TaTAP46 positively regulate drought tolerance in wheat by inhibiting PP2A activity [J]. J Integr Plant Biol., 2023, 65(9): 2056-2070.
[2]	Yuquan Wang, Chenyang Hao, Jun Zheng, Hongmei Ge, Yang Zhou, Zhengqiang Ma, and Xueyong Zhang. A haplotype block associated with thousand-kernel weight on chromosome 5DS in common wheat (Triticum aestivum L.) [J]. J Integr Plant Biol., 2015, 57(8): 662-672.
[3]	Bin Zhang, Xia Liu, Guangyao Zhao, Xinguo Mao, Ang Li, and Ruilian Jing. Molecular characterization and expression analysis of Triticum aestivum squamosa-promoter binding protein-box genes involved in ear development [J]. J Integr Plant Biol., 2014, 56(6): 571-581.
[4]	Hongwei Li, Gui Wang, Qi Zheng, Bin Li, Ruilian Jing, and Zhensheng Li. Genetic analysis of biomass and photosynthetic parameters in wheat grown in different light intensities [J]. J Integr Plant Biol., 2014, 56(6): 594-604.
[5]	Dong-Yao Gao, Zhao-Shi Xu, Yi He, Yong-Wei Sun, You-Zhi Ma, and Lan-Qin Xia. Functional analyses of an E3 ligase gene AIP2 from wheat in Arabidopsis revealed its roles in seed germination and pre-harvest sprouting [J]. J Integr Plant Biol., 2014, 56(5): 480-491.
[6]	Hongwei Li, Fanyun Lin, Gui Wang, Ruilian Jing, Qi Zheng, Bin Li, and Zhensheng Li. Quantitative Trait Loci Mapping of Dark-Induced Senescence in Winter Wheat (Triticum aestivum) [J]. J Integr Plant Biol., 2012, 54(1): 33-44.
[7]	Yu Takenouchi, Haruka Nakajima, Kengo Kanamaru, and Shigeo Takumi. Characterization of Three Homoeologous cDNAs Encoding Chloroplast-targeted Aminolevulinic Acid Dehydratase in Common Wheat [J]. J Integr Plant Biol., 2011, 53(12): 942-950.
[8]	Bohong Tian, and Hongjie Li. Variation of B Chromosome Associated with Tissue Culture in Wheat-rye Cross [J]. J Integr Plant Biol., 2009, 51(9): 834-839.
[9]	Yong-Mei Xia, Xue-Mei Gao and Qing-Jun Li. Identification and expression of floral organ homeotic genes from Alpinia oblongifolia (Zingiberaceae) [J]. J Integr Plant Biol., 2009, 51(2): 155-166.
[10]	Kun-Pu Zhang, Liang Zhao, Ji-Chun Tian, Guang-Feng Chen, Xiao-Ling Jiang and Bin Liu. A Genetic Map Constructed Using a Doubled Haploid Population Derived from Two Elite Chinese Common Wheat (Triticum aestivum L.) Varieties [J]. J Integr Plant Biol., 2008, 50(8): 941-950.
[11]	Yong-Qin Wang, Xiao-Li Wei, Hong-Lin Xu, Cheng-Lin Chai, Kun Meng, Hong-Li Zhai, Ai-Jun Sun, Yong-Gang Peng, Bin Wu, Gui-Fang Xiao and Zhen Zhu. Cell-wall Invertases from Rice are Differentially Expressed in Caryopsis during the Grain Filling Stage [J]. J Integr Plant Biol., 2008, 50(4): 466-474.
[12]	Bing Guan, Kai Wang, Baoliang Zhou, Wangzhen Guo, and Tianzhen Zhang. Establishment of a Multi-color GISH Technique to Simultaneously Discriminate the Three Interspecific Hybrid Genomes in Gossypium [J]. J Integr Plant Biol., 2008, 50(3): 345-351.
[13]	Hong Long, Zhong-Xia Qi, Xiao-Ming Sun, Cheng-Bin Chen, Xiu-Lan Li, Wen-Qin Song and Rui-Yang Chen. Characters of DNA Constitution in the Rye B Chromosome [J]. J Integr Plant Biol., 2008, 50(2): 183-189.
[14]	Mustafa YILDIZ∗. Two-Dimensional Electrophoretic Analysis of Soluble Leaf Proteins of a Salt-sensitive (Triticum aestivum) and a Salt-tolerant (T. durum) Cultivar in Response to NaCl Stress [J]. J Integr Plant Biol., 2007, 49(7): -.
[15]	Zhao Liu, Dayong Li and Xueyong Zhang. Genetic Relationships Among Five Basic Genomes St, E, A, B and D in Triticeae Revealed by Genomic Southern and in situ Hybridization [J]. J Integr Plant Biol., 2007, 49(7): -.

Hybridization of Aegilops caudata with Triticum durum, Triticum aestivum and the Detection of Alien Chromatins

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments