Selective Fractionation of Maize Sperm Cells by RCA I Sepharose 6MB Affinity  Chromatography

Abstract

Abstract: RCA I-Sepharose 6MB was employed for affinity chromatography of maize ( Zea mays L. ) sperm cells. The result indicates that the loading capacity of 0.1 mL RCA I-6MB is about 1.5 × 10 . If the amount of sperm cells is less than this capacity, about 70% of the sperm cells specifically bind to RCA I-6MB. 42.1% of the absorbed sperm cells can be released from RCA I-6MB in 0.2 mol/L galactose and recovered with less impurities and with some viability, which will facilitate further study on plant sperm cells. It remains to be elucidated whether there are two types of maize sperm cells different from each other in monosaccharide residues on their plasma membranes.

Key words: Sperm cell, Zea mays, RCA I-Sepharose 6MB, Affinity chromatography

XU Heng-Ping, JIN Cheng, YANG Shou-Jun, ZHANG Shu-Zheng and TSAO T H. Selective Fractionation of Maize Sperm Cells by RCA I Sepharose 6MB Affinity Chromatography[J]. J Integr Plant Biol., 1999, 41(9): -.

[1]	Senlin Xiao, Wei Song, Jinfeng Xing, Aiguo Su, Yanxin Zhao, Chunhui Li, Zi Shi, Zhiyong Li, Shuai Wang, Ruyang Zhang, Yuanrong Pei, Huabang Chen and Jiuran Zhao. ORF355 confers enhanced salinity stress adaptability to S-type cytoplasmic male sterility maize by modulating the mitochondrial metabolic homeostasis [J]. J Integr Plant Biol., 2023, 65(3): 656-673.
[2]	Kasra Sabermanesh, Luke R. Holtham, Jessey George, Ute Roessner, Berin A. Boughton, Sigrid Heuer, Mark Tester, Darren C. Plett, and Trevor P. Garnett. Transition from a maternal to external nitrogen source in maize seedlings [J]. J Integr Plant Biol., 2017, 59(4): 261-274.
[3]	Pengcheng Li, Zhongjuan Zhuang, Hongguang Cai, Shuai Cheng, Ayaz Ali Soomro, Zhigang Liu, Riliang Gu, Guohua Mi, Lixing Yuan, and Fanjun Chen. Use of genotype-environment interactions to elucidate the pattern of maize root plasticity to nitrogen deficiency [J]. J Integr Plant Biol., 2016, 58(3): 242-253.
[4]	Jilei Huang, Yan Ju, Xiangfeng Wang, Quan Zhang, and Sodmergen. A one-step rectification of sperm cell targeting ensures the success of double fertilization [J]. J Integr Plant Biol., 2015, 57(5): 496-503.
[5]	Yu-Rong Xie, Yenjit Raruang, Zhi-Yuan Chen, Robert L. Brown, and Thomas E. Cleveland. ZmGns, a maize class I β-1,3-glucanase, is induced by biotic stresses and possesses strong antimicrobial activity [J]. J Integr Plant Biol., 2015, 57(3): 271-283.
[6]	Domagoj Šimić, Hrvoje Lepeduš, Vlatka Jurković, Jasenka Antunović, and Vera Cesar. Quantitative genetic analysis of chlorophyll a fluorescence parameters in maize in the field environments [J]. J Integr Plant Biol., 2014, 56(7): 695-708.
[7]	Li-Min Zhang, Xiang-Guo Liu, Xin-Ning Qu, Ying Yu, Si-Ping Han, Yao Dou, Yao-Yao Xu, Hai-Chun Jing, and Dong-Yun Hao. Early Transcriptomic Adaptation to Na₂CO₃ Stress Altered the Expression of a Quarter of the Total Genes in the Maize Genome and Exhibited Shared and Distinctive Profiles with NaCl and High pH Stresses [J]. J Integr Plant Biol., 2013, 55(11): 1147-1165.
[8]	Chengsong Liao, Renyi Liu, Fusuo Zhang, Chunjian Li, and Xuexian Li. Nitrogen Under- and Over-supply Induces Distinct Protein Responses in Maize Xylem Sap [J]. J Integr Plant Biol., 2012, 54(6): 374-387.
[9]	Yuanzeng Zhao, Xiaomin Lu, Chaoxian Liu, Haiying Guan, Mei Zhang, Zhongfeng Li, Hongwei Cai, and Jinsheng Lai. Identification and Fine Mapping of rhm1 Locus for Resistance to Southern Corn Leaf Blight in Maize [J]. J Integr Plant Biol., 2012, 54(5): 321-329.
[10]	Zhuanfang Hao, Xinhai Li, Chuanxiao Xie, Jianfeng Weng, Mingshun Li, Degui Zhang, Xiaoling Liang, Lingling Liu, Sisi Liu, and Shihuang Zhang. Identification of Functional Genetic Variations Underlying Drought Tolerance in Maize Using SNP Markers [J]. J Integr Plant Biol., 2011, 53(8): 641-652.
[11]	Liangzheng Xu, Junfang Niu, Chunjian Li, and Fusuo Zhang. Growth, and Nitrogen Uptake and Flow in Maize Plants Affected by Root Growth Restriction [J]. J Integr Plant Biol., 2009, 51(7): 689-697.
[12]	Beibei Xue, Aying Zhang, and Mingyi Jiang. Involvement of Polyamine Oxidase in Abscisic Acid-induced Cytosolic Antioxidant Defense in Leaves of Maize [J]. J Integr Plant Biol., 2009, 51(3): 225-234.
[13]	Fan Lin, Yun Zhang and Ming-Yi Jiang. Alternative Splicing and Differential Expression of Two Transcripts of Nicotine Adenine Dinucleotide Phosphate Oxidase B Gene from Zea mays, [J]. J Integr Plant Biol., 2009, 51(3): 287-298.
[14]	Hui Huang, Song-Quan Song and Xian-Jin Wu. Response of Chinese Wampee Axes and Maize Embryos to Dehydration at Different Rates [J]. J Integr Plant Biol., 2009, 51(1): 67-74.
[15]	Lei Wang, Yanzhong Luo, Lan Zhang, Jun Zhao, Zhiqiu Hu, Yunliu Fan, Chunyi Zhang. Isolation and Characterization of a C-repeat Binding Transcription Factor from Maize [J]. J Integr Plant Biol., 2008, 50(8): 965-974.

Selective Fractionation of Maize Sperm Cells by RCA I Sepharose 6MB Affinity Chromatography

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments