J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (9): -.

• Research Articles •    

Selective Fractionation of Maize Sperm Cells by RCA I Sepharose 6MB Affinity Chromatography

XU Heng-Ping, JIN Cheng, YANG Shou-Jun, ZHANG Shu-Zheng and TSAO T H   

Abstract: RCA I-Sepharose 6MB was employed for affinity chromatography of maize ( Zea mays L. ) sperm cells. The result indicates that the loading capacity of 0.1 mL RCA I-6MB is about 1.5 × 10 . If the amount of sperm cells is less than this capacity, about 70% of the sperm cells specifically bind to RCA I-6MB. 42.1% of the absorbed sperm cells can be released from RCA I-6MB in 0.2 mol/L galactose and recovered with less impurities and with some viability, which will facilitate further study on plant sperm cells. It remains to be elucidated whether there are two types of maize sperm cells different from each other in monosaccharide residues on their plasma membranes.

Key words: Sperm cell, Zea mays, RCA I-Sepharose 6MB, Affinity chromatography

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