J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (5): -.

• Research Articles •    

Transcriptive Sites of RNA PolymeraseⅠin the Nucleolus of Triticum aestivum Revealed by BrUTP Labelling and Immunoelectron Microscopy

WANG Hua-Chun and XING Miao   

Abstract: The intact meristematic cells of Triticum aestivum L. were labelled with BrUTP and then detected with an anti-BrdU antibody and protein A-gold, and a large number of gold particles representing newly syn- thesized RP I transcripts were observed over the nucleolus of the cells. Most of the gold particles were located in the electron-dense area, which corresponds to DFC (dense fibrillar component), and the periphery of FC (fibrillar center), while the centre of FC and condensed chromatin showed few gold particles, suggesting that sites of RP I transcription are mainly located in DFC and the periphery of FC. Gold particles in DFC were frequently clustered and lined. The nucleolus-associated body (NAB) was often observed in the nucleus. It was a fibrillo-granular body closely associated with the nucleolus, and in which were concentrated gold particles representing the newly synthesized RNA.

Key words: BrUTP labelling, Immunoelectron microscopy, Nucleolus, Nucleohis-associated body, RNA polymerase I, Triticum aestivum

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