J Integr Plant Biol. ›› 1985, Vol. 27 ›› Issue (2): -.

• Research Articles •    

Studies on Nucleic Acid and Protein Synthesis and D evelopment of Male Gametnphyte of Clivia nobilis Cultured in Vitro

Tang Pei-hua and Zhu Ying-min   

Abstract: A simple method for culture male gametophyte (MG) of Clivia nobilis in vitro was established and the process of their development was observed. After research on dynamic of nucleic acid and protein synthesis of MG in various developmental stages by using of inhibitors and autoradiography authors found that DNA synthesis fro mrelease of tetrad to sperm only takes place in nucleus of interphase. There is no 3H-tymidine incorporation into vegetative nucleus (Vn), generative nucleus (Gn) or nucleus of sperm in 96 hours before dehiscence of anthers (BDA). The dynamic of protein synthesis is similar to the same of RNA’s. Both of them have three peaks and two intermissions. The 1st peak is in 12–9 days BDA. The 2nd is in 7–5 days BDA and intermitted from 48 hours BDA. The 3rd begins from the 1st hour after culture (AC) decleases at 6th hour AC and stops before 20th hour AC. The kind of inhibitor, the time and quanlity of treatment are affected the morphogenesis, showing the relationship to synthesis among DNA, RNA and protein and to the same between biomacromolecular and morphological development of MG.

Key words: Clivia nobilis, Male gametophyte, Culture, Nucleic acid and protein Synthesis

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