Molecular Cytogenetic Identification of Triticum aestivum-Secale cereale Substitution  and Addition Lines

Abstract

Abstract: Two substitution lines, designated as 930498 and 930483, and one addition line, designated as 930029, via Fo immature embryo culture of Triticum aestivum x octoploid triticale ( x Triti-cosecale Wittmack) were identified. Fluorescence in situ hybridization (FISH) using total genomic DNA of rye ( Secale cereale L. ) as probe corroborated the existence of rye chromosomes, further confirmed through chromosome paring at meiotic metaphase 1, C-banding and glutenin SDS- PAGE. The results demonstrated that the two substitution lines are ID/IR, and the addition line is also IR addition. Rye chromosomes that are distinct to the red-colored wheat chromosomes appear yellow-green at mitotic metaphase after FISH.

Key words: Triticum aestivum, Triticale, Secale cereale, Tissue culture, Substitution line, Addition line, Fluorescence in situ hybridization

LI Hong-Jie,ZHU Zhi-Qing, ZHANG Yan-Min, GUO Bei-Hai, WEN Yu-Xiang and JIA Xu. Molecular Cytogenetic Identification of Triticum aestivum-Secale cereale Substitution and Addition Lines[J]. J Integr Plant Biol., 1998, 40(1): -.

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Molecular Cytogenetic Identification of Triticum aestivum-Secale cereale Substitution and Addition Lines

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