J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (5): -.

• Research Articles •    

Asymmetric Somatic Hybridization Between Wheat (Triticum aestivum) and Bromus inermis

XIANG Feng-Ning, XIA Guang-Min, ZHOU Ai-Fen, CHEN Hui-Min, HUANG Yue and ZHAI Xiao-Ling   

Abstract: Asymmetric hybridization was conducted between wheat and Brorrats inermis keyss which is a distanfiy related intergeneric plant (belonging to different tribe) of wheat and possesses some favorable traits, such as resistant to cold, drought and disease. Protoplasts isolated from young embryo-derived calli of common wheat ( Triticura aestivum L., tv. 99P, (AABBDD), 2n = 42) were fused with UV-treated protoplasts isolated from young embryo-derived calli of Bromus inermis by PEG method. Three clones (No. 1 ~ No. 3) were regenerated from the fusion products and differentiated into albino seedlings. The clones and the seedlings were all verified as hybrids by chromosome counting, isozyme and RAPD analysis. Their isozyme and RAPD pattern contained the characteristic bands of both parents as well as new band(s). The chromosome numbers of albino were in the range of 42~54 with small chromosomes of Bromus inerm/s and chromosome fragments. The above results confirmed that hybrid albinos were obtained.

Key words: Triticum aestivum, Bromus inermis, UV-irradiation, Asymmetric protoplast fusion, Albino

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