Abstract: The roots of Anemone tomentosa (Maxim.) Pei (Ranunculaceae) have been used as traditional medicine for the treatment of dysentery, infantile malnutrition, carbuncles, etc. Ethyl acetate extract of the roots displaied stronger antifeedant activity against Leucania separata Walker. Eleven compounds were isolated by silica gel column chromatography from the extract and their structures were identified as 4,5-dimethoxyl-7- methyl-coumarin(1),4-methoxyl-5-methyl-6,7-methylenedioxy-coumarin (2), 4, 7-dimethoxyl-5-methyl-comnarin (3), oleanolic acid (4), oleanonic acid (5), 3-O-β-D-xylopyranosyl oleanolic acid (6),β-sitosterel (7), stigmasterol (8), daucosterol (9), 3-O-β-D-glucopyranosyl stigmasterol (10) and ergosterol peroxide (11) respectively on the basis of spectral evidence. Compounds 1 and 2 are new natural products.

Key words: Anemone tomentosa, Antifeedant activity, 4, 5-dimethoxyl-7-methyl-coumarin, 4-methoxyl-5-methyl-6, 7-methylenedioxycoumarin