Characterization of Genome and Chromosomes in Octoploid Wheat  wheatgrass Amphiploid Zhong 2 Using Fluorescence in situ               Hybridization and Chromosome Pairing Analysis

Abstract

Abstract: Genomic constitution of octoploid wheat-wheatgrass amphiploid Zhong 2 was analyzed by chromosome pairing and fluorescence in sim hybridization techniques. The results indicated that the octoploid wheatwheatgrass chromosomes in Zhong 2 were derived from the distant homologous genomes of wheatgrass ( Agropyron intermedium (Host) P.B. = Elytrigia intermedia (Host) Nevski = Thinotopyrum intermedium (Host) Barkworth and Dewey, and thew distant homologous genomes were not from the E geaome of T. elongatum 2x. Zhong 2 contained 12 wheatgrass chromosomes in which a pair of chromosomes was involved in translocation between wheatgrass and wheat chromosomes.

Key words: Common wheat, Wheatgrass, Octoploid wheat-wheatgrass, Chromosome pairing, fluorescence in situ hybridization

GAO Zhi, HAN Fang-Pu,HE Meng-Yuan,MA You-Zhi and XIN Zhi-Yong. Characterization of Genome and Chromosomes in Octoploid Wheat wheatgrass Amphiploid Zhong 2 Using Fluorescence in situ Hybridization and Chromosome Pairing Analysis[J]. J Integr Plant Biol., 1999, 41(1): -.

[1]	Guoguo Lv, Yixiao Zhang, Lin Ma, Xiangning Yan, Mingjie Yuan, Jianhui Chen, Yongzhen Cheng, Xi Yang, Qi Qiao, Leilei Zhang, Mohsin Niaz, Xiaonan Sun, Qijun Zhang, Shaobin Zhong and Feng Chen. A cell wall invertase modulates resistance to fusarium crown rot and sharp eyespot in common wheat [J]. J Integr Plant Biol., 2023, 65(7): 1814-1825.
[2]	Xuening Wei, Yuyan Li, Xiuliang Zhu, Xin Liu, Xingguo Ye, Miaoping Zhou and Zengyan Zhang. The GATA transcription factor TaGATA1 recruits demethylase TaELF6‐A1 and enhances seed dormancy in wheat by directly regulating TaABI5 [J]. J Integr Plant Biol., 2023, 65(5): 1262-1276.
[3]	Liqing Gu, Bo Wei, Renchun Fan, Xu Jia, Xianping Wang and Xiangqi Zhang. Development, identification and utilization of introgression lines using Chinese endemic and synthetic wheat as donors [J]. J Integr Plant Biol., 2015, 57(8): 688-697.
[4]	Qing-Ming Sun, Rong-Hua Zhou, Li-Feng Gao, Guang-Yao Zhao, and Ji-Zeng Jia. The Characterization and Geographical Distribution of the Genes Responsible for Vernalization Requirement in Chinese Bread Wheat [J]. J Integr Plant Biol., 2009, 51(4): 423-432.
[5]	Zhong-Wei Yuan, Qi-Jiao Chen, Lian-Quan Zhang, Ze-Hong Yan,You-Liang Zheng and Deng-Cai Liu. Molecular Characterization of Two Silenced y-type Genes for Glu-B1 in Triticum aestivum ssp. yunnanese and ssp. tibetanum [J]. J Integr Plant Biol., 2009, 51(1): 93-99.
[6]	Kun-Pu Zhang, Liang Zhao, Ji-Chun Tian, Guang-Feng Chen, Xiao-Ling Jiang and Bin Liu. A Genetic Map Constructed Using a Doubled Haploid Population Derived from Two Elite Chinese Common Wheat (Triticum aestivum L.) Varieties [J]. J Integr Plant Biol., 2008, 50(8): 941-950.
[7]	Zhi-Jun Cheng, Minoru Murata, Sodmergen, Xiao-Mei Li, Hai Nian and Jian-Min Wan. Effects on Genome Constitution and Novel Cell Wall Formation Caused by the Addition of 5RS Rye Chromosome to Common Wheat [J]. J Integr Plant Biol., 2008, 50(4): 503-509.
[8]	Hong Long, Zhong-Xia Qi, Xiao-Ming Sun, Cheng-Bin Chen, Xiu-Lan Li, Wen-Qin Song and Rui-Yang Chen. Characters of DNA Constitution in the Rye B Chromosome [J]. J Integr Plant Biol., 2008, 50(2): 183-189.
[9]	Rui Luo, Chao Wang and Daming Zhang. Variations of 18S rDNA Loci Among Six Populations of Paeonia obovata Maxim. (Paeoniaceae) Revealed by Fluorescence In Situ Hybridization [J]. J Integr Plant Biol., 2006, 48(5): -.
[10]	Zhao Liu, Wei Yue, Yu-Shen Dong and Xue-Yong Zhang. Identification and Preliminary Analysis of Several Centromere-associated Bacterial Artificial Chromosome Clones from a Diploid Wheat Library [J]. J Integr Plant Biol., 2006, 48(3): 348-363.
[11]	Wen-Hui WEI, Wan-Peng ZHAO, Li-Jun WANG, Bo CHEN, Yun-Chang LI and Yun-Chun SONG. Karyotyping of Brassica napus L. Based on C0t-1 DNA Banding by Fluorescence In Situ Hybridization [J]. J Integr Plant Biol., 2005, 47(12): 1479-1484.
[12]	Zhi-Yong XIONG, Yong LIU, Yong-Gang HE, Yun-Chun SONG, Ke-Xiu LI and Guan-Yuan HE. Heterozygosity of Knob-Associated Tandem Repeats and Knob Instability in Mitotic Chromosomes of Zea (Zea mays L. and Z. diploperennis Iltis Doebley) [J]. J Integr Plant Biol., 2005, 47(11): 1345-1351.
[13]	CHENG Ai-Xia, XIA Guang-Min, CHEN Hui-Min. DNA Transfer from Wild Millet to Common Wheat by Asymmetric Somatic Hybridization [J]. J Integr Plant Biol., 2004, 46(9): 1114-1121.
[14]	BAI Jian-Rong, JIA Xu, LIU Kun-Fan, WANG Dao-Wen. Cloning and Characterization of the Coding Sequences of the 1Ay High Molecular Weight Glutenin Subunit Genes from Triticum urartu [J]. J Integr Plant Biol., 2004, 46(4): 463-471.
[15]	LIU Deng Cai, LAN Xiu Jin, YANG Zu Jun, ZHENG You Liang, WEI Yu Ming, ZHOU Yong Hong. A Unique Aegilops tauschii Genotype Needless to Immature Embryo Culture in Cross with Wheat [J]. J Integr Plant Biol., 2002, 44(6): 708-713.

Characterization of Genome and Chromosomes in Octoploid Wheat wheatgrass Amphiploid Zhong 2 Using Fluorescence in situ Hybridization and Chromosome Pairing Analysis

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments