J Integr Plant Biol. ›› 1985, Vol. 27 ›› Issue (3): -.

• Research Articles •    

Callus Formation and Morphogenesis of Cultured Mustard (Brassica juncea) Protoplasts

Li Wen-bin, Chen Zhi-zheng, Zhang Da-wei, Chen Zheng-hua   

Abstract: Protoplasts from mustard seedling cotyledons were suspended in Nitsch medium and cultured at 26℃ under low intensity illumination. When colonies were observed in the cultures, fresh medium (3% sucrose replaced mannitol) was added by equalvolume. When protoplasts developed into small callus the cultures were transfered to modified MS agar medium, in which some of them produced roots while an others gave rise to green spots. Shoot-differentiation experiment is in progress.

Key words: Protoplast, Callus, Brassica juncea

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