J Integr Plant Biol. ›› 2016, Vol. 58 ›› Issue (4): 426-439.DOI: 10.1111/jipb.12446

• Research Articles • Previous Articles    

Bacillus cereus AR156 primes induced systemic resistance by suppressing miR825/825* and activating defense related genes in Arabidopsis

Dongdong Niu1,2, Jing Xia3,4, Chunhao Jiang1, Beibei Qi1, Xiaoyu Ling1, Siyuan Lin1, Weixiong Zhang3, Jianhua Guo1*, Hailing Jin2* and Hongwei Zhao1*   

  1. 1Department of Plant Pathology, College of Plant Protection, Nanjing Agriculture University, Nanjing, China
    2Department of Plant Pathology and Microbiology, Center for Plant Cell Biology and Institute for Integrative Genome Biology, University of California, Riverside, California, CA-92521, USA
    3Department of Computer Science and Engineering, Washington University in St Louis, St Louis, MO 63130, USA
    4The Institute for Systems Biology, Jianghan University, Wuhan 430056, China

Abstract:

Small RNAs play an important role in plant immune responses. However, their regulatory function in induced systemic resistance (ISR) is nascent. Bacillus cereus AR156 is a plant growth-promoting rhizobacterium that induces ISR in Arabidopsis against bacterial infection. Here, by comparing small RNA profiles of Pseudomonas syringae pv. tomato (Pst) DC3000-infected Arabidopsis with and without AR156 pretreatment, we identified a group of Arabidopsis microRNAs (miRNAs) that are differentially regulated by AR156 pretreatment. miR825 and miR825* are two miRNA generated from a single miRNA gene. Northern blot analysis indicated that they were significantly downregulated in Pst DC3000-infected plants pretreated with AR156, in contrast to the plants without AR156 pretreatment. miR825 targets two ubiquitin-protein ligases, while miR825* targets toll-interleukin-like receptor (TIR)-nucleotide binding site (NBS) and leucine-rich repeat (LRR) type resistance (R) genes. The expression of these target genes negatively correlated with the expression of miR825 and miR825*. Moreover, transgenic plants showing reduced expression of miR825 and miR825* displayed enhanced resistance to Pst DC3000 infection, whereas transgenic plants overexpressing miR825 and miR825* were more susceptible. Taken together, our data indicates that Bacillus cereus AR156 pretreatment primes ISR to Pst infection by suppressing miR825 and miR825* and activating the defense related genes they targeted.

Key words: Induced systemic resistance, ISR, microRNA, plant innate immunity, small RNA

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