J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (12): 1071-1079.DOI: 10.1111/j.1744-7909.2009.00887.x

Special Issue: Cell Biology and Functions

• Cell and Developmental Biology • Previous Articles     Next Articles

Identification of MicroRNAs in Wild Soybean (Glycine soja)

Rui Chen, Zheng Hu and Hui Zhang*   

  1. The National Key Facilities for Crop Genetic Resources and Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2009-06-11 Accepted:2009-09-09 Published:2009-12-01
  • About author: *Author for correspondence
    Tel (Fax): +86 10 6218 6649; E-mail: zhang_hui@mail.caas.net.cn

Abstract:

MicroRNAs (miRNAs) play important roles in post-transcriptional gene silencing by directing target mRNA cleavage or translational inhibition. Currently, hundreds of miRNAs have been identified in plants, but no report has been published of wild soybean (Glycine soja Sieb). We constructed a small-RNA library consisting of 2 880 sequences with high quality, in which 1 347 were 19–24 nt in length. By utilizing the miRNA, Rfam and domesticated soybean expressed sequence tag database, we have analyzed and predicted the secondary structure of these small RNAs. As a result, 15 conserved miRNA candidates belonging to eight different families and nine novel miRNA candidates comprising eight families were identified in wild soybean seedlings. All these miRNA candidates were validated by northern blot and the novel candidates expressed in a tissue-specific manner. Furthermore, putative target genes were predicted for novel miRNA candidates and two of them were verified by 5'-rapid amplification of cDNA ends experiments. These results provided useful information for miRNA research in wild soybean and plants.
 

Chen R, Hu Z, Zhang H (2009). Identification of microRNAs in wild soybean (Glycine soja). J. Integr. Plant Biol. 51(12), 1071–1079.

Key words: microRNAs, plants, wild soybean.

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