J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (3): 692-702.DOI: 10.1111/jipb.13396

• Abiotic Stress Responses • Previous Articles     Next Articles

SUMOylation facilitates the assembly of a Nuclear Factor-Y complex to enhance thermotolerance in Arabidopsis

Junwen Huang, Junjie Huang, Qiyi Feng, Yaqiao Shi, Feige Wang, Kaiyong Zheng, Qize Huang, Jieming Jiang, Siyi Luo, Yun Xie, Danlu Han, Jianbin Lai* and Chengwei Yang*   

  1. Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou 510631, China
    *Correspondences: Chengwei Yang (yangchw@scnu.edu.cn, Dr. Yang is fully responsible for the distribution of all materials associated with this article); Jianbin Lai (20141062@m.scnu.edu.cn)
  • Received:2022-06-11 Accepted:2022-10-21 Online:2022-10-25 Published:2023-03-01

Abstract: Heat stress (HS) has serious negative effects on plant development and has become a major threat to agriculture. A rapid transcriptional regulatory cascade has evolved in plants in response to HS. Nuclear Factor-Y (NF-Y) complexes are critical for this mechanism, but how NF-Y complexes are regulated remains unclear. In this study, we identified NF-YC10 (NF-Y subunit C10), a central regulator of the HS response in Arabidopsis thaliana, as a substrate of SUMOylation, an important post-translational modification. Biochemical analysis showed that the SUMO ligase SIZ1 (SAP AND MIZ1 DOMAIN-CONTAINING LIGASE1) interacts with NF-YC10 and enhances its SUMOylation during HS. The SUMOylation of NF-YC10 facilitates its interaction with and the nuclear translocation of NF-YB3, in which the SUMO interaction motif (SIM) is essential for its efficient association with NF-YC10. Further functional analysis indicated that the SUMOylation of NF-YC10 and the SIM of NF-YB3 are critical for HS-responsive gene expression and plant thermotolerance. These findings uncover a role for the SIZ1-mediated SUMOylation of NF-YC10 in NF-Y complex assembly under HS, providing new insights into the role of a post-translational modification in regulating transcription during abiotic stress responses in plants.

Key words: Arabidopsis, complex assembly, heat stress, Nuclear Factor-Y, SIZ1, SUMOylation

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