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Genomic analysis of Nypa fruticans elucidates its intertidal adaptations and early palm evolution
Weihong Wu, Xiao Feng, Nan Wang, Shao Shao, Min Liu, Fa Si, Linhao Chen, Chuanfeng Jin, Shaohua Xu, Zixiao Guo, Cairong Zhong, Suhua Shi and Ziwen He
doi: 10.1111/jipb.13625
Version of Record online: 19 February 2024
An alfalfa MYB-like transcriptional factor MsMYBH positively regulates alfalfa seedling drought resistance and undergoes MsWAV3-mediated degradation
Kun Shi, Jia Liu, Huan Liang, Hongbin Dong, Jinli Zhang, Yuanhong Wei, Le Zhou, Shaopeng Wang, Jiahao Zhu, Mingshu Cao, Chris S. Jones, Dongmei Ma and Zan Wang
doi: 10.1111/jipb.13626
Version of Record online: 15 February 2024
CRISPR/CasΦ2-mediated gene editing in wheat and rye
Sanzeng Zhao, Xueying Han, Yachen Zhu, Yuwei Han, Huiyun Liu, Zhen Chen, Huifang Li, Dan Wang, Chaofan Tian, Yuting Yuan, Yajie Guo, Xiaomin Si, Daowen Wang and Xiang Ji
doi: 10.1111/jipb.13624
Version of Record online: 13 February 2024
Reactive oxygen species: Multidimensional regulators of plant adaptation to abiotic stress and development
Pengtao Wang, Wen-Cheng Liu, Chao Han, Situ Wang, Ming-Yi Bai and Chun-Peng Song
doi: 10.1111/jipb.13601
Version of Record online: 29 January 2024
Breeding maize of ideal plant architecture for high-density planting tolerance through modulating shade avoidance response and beyond
Fereshteh Jafari, Baobao Wang, Haiyang Wang and Junjie Zou
doi: 10.1111/jipb.13603
Version of Record online: 29 January 2024
A historical review of hybrid rice breeding
Xiaoming Zheng, Fei Wei, Cheng Cheng and Qian Qian
doi: 10.1111/jipb.13598
Version of Record online: 22 January 2024
Chromosomal-level genome and metabolome analyses of highly heterozygous allohexaploid Dendrocalamus brandisii elucidate shoot quality and developmental characteristics
Jutang Jiang, Zeyu Zhang, Yucong Bai, Xiaojing Wang, Yuping Dou, Ruiman Geng, Chongyang Wu, Hangxiao Zhang, Cunfu Lu, Lianfeng Gu and Jian Gao
doi: 10.1111/jipb.13592
Version of Record online: 12 January 2024
Wood of trees: Cellular structure, molecular formation, and genetic engineering
Yingying Zhu and Laigeng Li
doi: 10.1111/jipb.13589
Version of Record online: 09 January 2024
The dual‐action mechanism of Arabidopsis cryptochromes
Gao‐Ping Qu, Bochen Jiang and Chentao Lin
doi: 10.1111/jipb.13578
Version of Record online: 02 January 2024
Plant virology in the 21st century in China: Recent advances and future directions
Jianguo Wu, Yongliang Zhang, Fangfang Li, Xiaoming Zhang, Jian Ye, Taiyun Wei, Zhenghe Li, Xiaorong Tao, Feng Cui, Xianbing Wang, Lili Zhang, Fei Yan, Shifang Li, Yule Liu, Dawei Li, Xueping Zhou and Yi Li
doi: 10.1111/jipb.13580
Version of Record online: 02 January 2024
Phase separation of S‐RNase promotes self‐incompatibility in Petunia hybrida
Huayang Tian, Hongkui Zhang, Huaqiu Huang, Yu'e Zhang and Yongbiao Xue
doi: 10.1111/jipb.13584
Version of Record online: 02 January 2024
A centromere map based on super pan-genome highlights the structure and function of rice centromeres
Yang Lv, Congcong Liu, Xiaoxia Li, Yueying Wang, Huiying He, Wenchuang He, Wu Chen, Longbo Yang, Xiaofan Dai, Xinglan Cao, Xiaoman Yu, Jiajia Liu, Bin Zhang, Hua Wei, Hong Zhang, Hongge Qian, Chuanlin Shi, Yue Leng, Xiangpei Liu, Mingliang Guo, Xianmeng Wang, Zhipeng Zhang, Tianyi Wang, Bintao Zhang, Qiang Xu, Yan Cui, Qianqian Zhang, Qiaoling Yuan, Noushin Jahan, Jie Ma, Xiaoming Zheng, Yongfeng Zhou, Qian Qian, Longbiao Guo and Lianguang Shang
J Integr Plant Biol 2024, 66 (2): 196-207.  
doi: 10.1111/jipb.13607
Abstract (Browse 121)  |   Save
Rice (Oryza sativa) is a significant crop worldwide with a genome shaped by various evolutionary factors. Rice centromeres are crucial for chromosome segregation, and contain some unreported genes. Due to the diverse and complex centromere region, a comprehensive understanding of rice centromere structure and function at the population level is needed. We constructed a high-quality centromere map based on the rice super pan-genome consisting of a 251-accession panel comprising both cultivated and wild species of Asian and African rice. We showed that rice centromeres have diverse satellite repeat CentO, which vary across chromosomes and subpopulations, reflecting their distinct evolutionary patterns. We also revealed that long terminal repeats (LTRs), especially young Gypsy-type LTRs, are abundant in the peripheral CentO-enriched regions and drive rice centromere expansion and evolution. Furthermore, high-quality genome assembly and complete telomere-to-telomere (T2T) reference genome enable us to obtain more centromeric genome information despite mapping and cloning of centromere genes being challenging. We investigated the association between structural variations and gene expression in the rice centromere. A centromere gene, OsMAB, which positively regulates rice tiller number, was further confirmed by expression quantitative trait loci, haplotype analysis and clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 methods. By revealing the new insights into the evolutionary patterns and biological roles of rice centromeres, our finding will facilitate future research on centromere biology and crop improvement.
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Angiosperm-wide analysis of fruit and ovary evolution aided by a new nuclear phylogeny supports association of the same ovary type with both dry and fleshy fruits
Yezi Xiang, Taikui Zhang, Yiyong Zhao, Hongjin Dong, Hongyi Chen, Yi Hu, Chien‐Hsun Huang, Jun Xiang and Hong Ma
J Integr Plant Biol 2024, 66 (2): 228-251.  
doi: 10.1111/jipb.13618
Abstract (Browse 11)  |   Save
Fruit functions in seed protection and dispersal and belongs to many dry and fleshy types, yet their evolutionary pattern remains unclear in part due to uncertainties in the phylogenetic relationships among several orders and families. Thus we used nuclear genes of 502 angiosperm species representing 231 families to reconstruct a well supported phylogeny, with resolved relationships for orders and families with previously uncertain placements. Using this phylogeny as a framework, molecular dating supports a Triassic origin of the crown angiosperms, followed by the emergence of most orders in the Jurassic and Cretaceous and their rise to ecological dominance during the Cretaceous Terrestrial Revolution. The robust phylogeny allowed an examination of the evolutionary pattern of fruit and ovary types, revealing a trend of parallel carpel fusions during early diversifications in eudicots, monocots, and magnoliids. Moreover, taxa in the same order or family with the same ovary type can develop either dry or fleshy fruits with strong correlations between specific types of dry and fleshy fruits; such associations of ovary, dry and fleshy fruits define several ovary-fruit “modules” each found in multiple families. One of the frequent modules has an ovary containing multiple ovules, capsules and berries, and another with an ovary having one or two ovules, achenes (or other single-seeded dry fruits) and drupes. This new perspective of relationships among fruit types highlights the closeness of specific dry and fleshy fruit types, such as capsule and berry, that develop from the same ovary type and belong to the same module relative to dry and fleshy fruits of other modules (such as achenes and drupes). Further analyses of gene families containing known genes for ovary and fruit development identified phylogenetic nodes with multiple gene duplications, supporting a possible role of whole-genome duplications, in combination with climate changes and animal behaviors, in angiosperm fruit and ovary diversification.
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Increased long-distance and homo-trans interactions related to H3K27me3 in Arabidopsis hybrids
Zhaoxu Gao, Yanning Su, Le Chang, Guanzhong Jiao, Yang Ou, Mei Yang, Chao Xu, Pengtao Liu, Zejia Wang, Zewen Qi, Wenwen Liu, Linhua Sun, Guangming He, Xing Wang Deng and Hang He
J Integr Plant Biol 2024, 66 (2): 208-227.  
doi: 10.1111/jipb.13620
Abstract (Browse 8)  |   Save
In plants, the genome structure of hybrids changes compared with their parents, but the effects of these changes in hybrids remain elusive. Comparing reciprocal crosses between Col×C24 and C24×Col in Arabidopsis using high-throughput chromosome conformation capture assay (Hi-C) analysis, we found that hybrid three-dimensional (3D) chromatin organization had more long-distance interactions relative to parents, and this was mainly located in promoter regions and enriched in genes with heterosis-related pathways. The interactions between euchromatin and heterochromatin were increased, and the compartment strength decreased in hybrids. In compartment domain (CD) boundaries, the distal interactions were more in hybrids than their parents. In the hybrids of CURLY LEAF (clf) mutants clfCol×clfC24 and clfC24×clfCol, the heterosis phenotype was damaged, and the long-distance interactions in hybrids were fewer than in their parents with lower H3K27me3. ChIP-seq data revealed higher levels of H3K27me3 in the region adjacent to the CD boundary and the same interactional homo-trans sites in the wild-type (WT) hybrids, which may have led to more long-distance interactions. In addition, the differentially expressed genes (DEGs) located in the boundaries of CDs and loop regions changed obviously in WT, and the functional enrichment for DEGs was different between WT and clf in the long-distance interactions and loop regions. Our findings may therefore propose a new epigenetic explanation of heterosis in the Arabidopsis hybrids and provide new insights into crop breeding and yield increase.
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The Ti-TAN plasmid toolbox for TurboID-based proximity labeling assays in Nicotiana benthamiana
Huang Tan, Yu Zhou, Erik Dinius and Rosa Lozano‐Durán
J Integr Plant Biol 2024, 66 (2): 166-168.  
doi: 10.1111/jipb.13610
Abstract (Browse 1)  |   Save
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IbNIEL-mediated degradation of IbNAC087 regulates jasmonic acid-dependent salt and drought tolerance in sweet potato
Xu Li, Zhen Wang, Sifan Sun, Zhuoru Dai, Jun Zhang, Wenbin Wang, Kui Peng, Wenhao Geng, Shuanghong Xia, Qingchang Liu, Hong Zhai, Shaopei Gao, Ning Zhao, Feng Tian, Huan Zhang and Shaozhen He
J Integr Plant Biol 2024, 66 (2): 176-195.  
doi: 10.1111/jipb.13612
Abstract (Browse 1)  |   Save
Sweet potato (Ipomoea batatas [L.] Lam.) is a crucial staple and bioenergy crop. Its abiotic stress tolerance holds significant importance in fully utilizing marginal lands. Transcriptional processes regulate abiotic stress responses, yet the molecular regulatory mechanisms in sweet potato remain unclear. In this study, a NAC (NAM, ATAF1/2, and CUC2) transcription factor, IbNAC087, was identified, which is commonly upregulated in salt- and drought-tolerant germplasms. Overexpression of IbNAC087 increased salt and drought tolerance by increasing jasmonic acid (JA) accumulation and activating reactive oxygen species (ROS) scavenging, whereas silencing this gene resulted in opposite phenotypes. JA-rich IbNAC087-OE (overexpression) plants exhibited more stomatal closure than wild-type (WT) and IbNAC087-Ri plants under NaCl, polyethylene glycol, and methyl jasmonate treatments. IbNAC087 functions as a nuclear transcriptional activator and directly activates the expression of the key JA biosynthesis-related genes lipoxygenase (IbLOX) and allene oxide synthase (IbAOS). Moreover, IbNAC087 physically interacted with a RING-type E3 ubiquitin ligase NAC087-INTERACTING E3 LIGASE (IbNIEL), negatively regulating salt and drought tolerance in sweet potato. IbNIEL ubiquitinated IbNAC087 to promote 26S proteasome degradation, which weakened its activation on IbLOX and IbAOS. The findings provide insights into the mechanism underlying the IbNIEL-IbNAC087 module regulation of JA-dependent salt and drought response in sweet potato and provide candidate genes for improving abiotic stress tolerance in crops.
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Post-transcriptional regulation of grain weight and shape by the RBP-A-J-K complex in rice
Ding Ren, Hui Liu, Xuejun Sun, Fan Zhang, Ling Jiang, Ying Wang, Ning Jiang, Peiwen Yan, Jinhao Cui, Jinshui Yang, Zhikang Li, Pingli Lu and Xiaojin Luo
J Integr Plant Biol 2024, 66 (1): 66-85.  
doi: 10.1111/jipb.13583
Abstract (Browse 112)  |   Save
RNA-binding proteins (RBPs) are components of the post-transcriptional regulatory system, but their regulatory effects on complex traits remain unknown. Using an integrated strategy involving map-based cloning, functional characterizations, and transcriptomic and population genomic analyses, we revealed that RBP-K (LOC_Os08g23120), RBP-A (LOC_Os11g41890), and RBP-J (LOC_Os10g33230) encode proteins that form an RBP-A-J-K complex that negatively regulates rice yield-related traits. Examinations of the RBP-A-J-K complex indicated RBP-K functions as a relatively non-specific RBP chaperone that enables RBP-A and RBP-J to function normally. Additionally, RBP-J most likely affects GA pathways, resulting in considerable increases in grain and panicle lengths, but decreases in grain width and thickness. In contrast, RBP-A negatively regulates the expression of genes most likely involved in auxin-regulated pathways controlling cell wall elongation and carbohydrate transport, with substantial effects on the rice grain filling process as well as grain length and weight. Evolutionarily, RBP-K is relatively ancient and highly conserved, whereas RBP-J and RBP-A are more diverse. Thus, the RBP-A-J-K complex may represent a typical functional model for many RBPs and protein complexes that function at transcriptional and post-transcriptional levels in plants and animals for increased functional consistency, efficiency, and versatility, as well as increased evolutionary potential. Our results clearly demonstrate the importance of RBP-mediated post-transcriptional regulation for the diversity of complex traits. Furthermore, rice grain yield and quality may be enhanced by introducing various complete or partial loss-of-function mutations to specific RBP genes using clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 technology and by exploiting desirable natural tri-genic allelic combinations at the loci encoding the components of the RBP-A-J-K complex through marker-assisted selection.
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Trade-offs between the accumulation of cuticular wax and jasmonic acid-mediated herbivory resistance in maize
Jiong Liu, Lu Li, Zhilong Xiong, Christelle A.M. Robert, Baozhu Li, Shan He, Wenjie Chen, Jiasheng Bi, Guanqing Zhai, Siyi Guo, Hui Zhang, Jieping Li, Shutang Zhou, Xi Zhang and Chun‐Peng Song
J Integr Plant Biol 2024, 66 (1): 143-159.  
doi: 10.1111/jipb.13586
Abstract (Browse 118)  |   Save
Plants have evolved complex physical and chemical defense systems that allow them to withstand herbivory infestation. Composed of a complex mixture of very-long-chain fatty acids (VLCFAs) and their derivatives, cuticular wax constitutes the first physical line of defense against herbivores. Here, we report the function of Glossy 8 (ZmGL8), which encodes a 3-ketoacyl reductase belonging to the fatty acid elongase complex, in orchestrating wax production and jasmonic acid (JA)-mediated defenses against herbivores in maize (Zea mays). The mutation of GL8 enhanced chemical defenses by activating the JA-dependent pathway. We observed a trade-off between wax accumulation and JA levels across maize glossy mutants and 24 globally collected maize inbred lines. In addition, we demonstrated that mutants defective in cuticular wax biosynthesis in Arabidopsis thaliana and maize exhibit enhanced chemical defenses. Comprehensive transcriptomic and lipidomic analyses indicated that the gl8 mutant confers chemical resistance to herbivores by remodeling VLCFA-related lipid metabolism and subsequent JA biosynthesis and signaling. These results suggest that VLCFA-related lipid metabolism has a critical role in regulating the trade-offs between cuticular wax and JA-mediated chemical defenses.
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Unveiling a half-century mystery of molecular bases for three-line hybrid rice breeding system
Xiaoming Zheng and Qian Qian
J Integr Plant Biol 2024, 66 (1): 3-6.  
doi: 10.1111/jipb.13590
Abstract (Browse 81)  |   Save
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Low temperature-mediated repression and far-red light-mediated induction determine morning FLOWERING LOCUS T expression levels
Hayeon Kim, Hye Won Kang, Dae Yeon Hwang, Nayoung Lee, Akane Kubota, Takato Imaizumi and Young Hun Song
J Integr Plant Biol 2024, 66 (1): 103-120.  
doi: 10.1111/jipb.13595
Abstract (Browse 76)  |   Save
In order to flower in the appropriate season, plants monitor light and temperature changes and alter downstream pathways that regulate florigen genes such as Arabidopsis (Arabidopsis thaliana) FLOWERING LOCUS T (FT). In Arabidopsis, FT messenger RNA levels peak in the morning and evening under natural long-day conditions (LDs). However, the regulatory mechanisms governing morning FT induction remain poorly understood. The morning FT peak is absent in typical laboratory LDs characterized by high red:far-red light (R:FR) ratios and constant temperatures. Here, we demonstrate that ZEITLUPE (ZTL) interacts with the FT repressors TARGET OF EATs (TOEs), thereby repressing morning FT expression in natural environments. Under LDs with simulated sunlight (R:FR = 1.0) and daily temperature cycles, which are natural LD-mimicking environmental conditions, FT transcript levels in the ztl mutant were high specifically in the morning, a pattern that was mirrored in the toe1 toe2 double mutant. Low night-to-morning temperatures increased the inhibitory effect of ZTL on morning FT expression by increasing ZTL protein levels early in the morning. Far-red light counteracted ZTL activity by decreasing its abundance (possibly via phytochrome A (phyA)) while increasing GIGANTEA (GI) levels and negatively affecting the formation of the ZTL–GI complex in the morning. Therefore, the phyA-mediated high-irradiance response and GI play pivotal roles in morning FT induction. Our findings suggest that the delicate balance between low temperature-mediated ZTL activity and the far-red light-mediated functions of phyA and GI offers plants flexibility in fine-tuning their flowering time by controlling FT expression in the morning.
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The advantages of crosstalk during the evolution of the BZR1-ARF6-PIF4 (BAP) module
Runjie Diao, Mengru Zhao, Yannan Liu, Zhenhua Zhang and Bojian Zhong
J Integr Plant Biol 2023, 65 (12): 2631-2644.  
doi: 10.1111/jipb.13554
Abstract (Browse 131)  |   Save
The BAP module, comprising BRASSINAZOLE RESISTANT 1 (BZR1), AUXIN RESPONSE FACTOR 6 (ARF6), and PHYTOCHROME-INTERACTING FACTOR 4 (PIF4), functions as a molecular hub to orchestrate plant growth and development. In Arabidopsis thaliana, components of the BAP module physically interact to form a complex system that integrates light, brassinosteroid (BR), and auxin signals. Little is known about the origin and evolution of the BAP module. Here, we conducted comparative genomic and transcriptomic analyses to investigate the evolution and functional diversification of the BAP module. Our results suggest that the BAP module originated in land plants and that the ζ, ε, and γ whole-genome duplication/triplication events contributed to the expansion of BAP module components in seed plants. Comparative transcriptomic analysis suggested that the prototype BAP module arose in Marchantia polymorpha, experienced stepwise evolution, and became established as a mature regulatory system in seed plants. We developed a formula to calculate the signal transduction productivity of the BAP module and demonstrate that more crosstalk among components enables higher signal transduction efficiency. Our results reveal the evolutionary history of the BAP module and provide insights into the evolution of plant signaling networks and the strategies employed by plants to integrate environmental and endogenous signals.
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Identification of natural allelic variation in TTL1 controlling thermotolerance and grain size by a rice super pan-genome
Yarong Lin, Yiwang Zhu, Yuchao Cui, Hongge Qian, Qiaoling Yuan, Rui Chen, Yan Lin, Jianmin Chen, Xishi Zhou, Chuanlin Shi, Huiying He, Taijiao Hu, Chenbo Gu, Xiaoman Yu, Xiying Zhu, Yuexing Wang, Qian Qian, Cuijun Zhang, Feng Wang and Lianguang Shang
J Integr Plant Biol 2023, 65 (12): 2541-2551.  
doi: 10.1111/jipb.13568
Abstract (Browse 141)  |   Save
Continuously increasing global temperatures present great challenges to food security. Grain size, one of the critical components determining grain yield in rice (Oryza sativa L.), is a prime target for genetic breeding. Thus, there is an immediate need for genetic improvement in rice to maintain grain yield under heat stress. However, quantitative trait loci (QTLs) endowing heat stress tolerance and grain size in rice are extremely rare. Here, we identified a novel negative regulator with pleiotropic effects, Thermo-Tolerance and grain Length 1 (TTL1), from the super pan-genomic and transcriptomic data. Loss-of-function mutations in TTL1 enhanced heat tolerance, and caused an increase in grain size by coordinating cell expansion and proliferation. TTL1 was shown to function as a transcriptional regulator and localized to the nucleus and cell membrane. Furthermore, haplotype analysis showed that hapL and hapS of TTL1 were obviously correlated with variations of thermotolerance and grain size in a core collection of cultivars. Genome evolution analysis of available rice germplasms suggested that TTL1 was selected during domestication of the indica and japonica rice subspecies, but still had much breeding potential for increasing grain length and thermotolerance. These findings provide insights into TTL1 as a novel potential target for the development of high-yield and thermotolerant rice varieties.
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Paternally imprinted LATE-FLOWERING2 transcription factor contributes to paternal-excess interploidy hybridization barriers in wheat
Guanghui Yang, Man Feng, Kuohai Yu, Guangxian Cui, Yan Zhou, Lv Sun, Lulu Gao, Yumei Zhang, Huiru Peng, Yingyin Yao, Zhaorong Hu, Vincenzo Rossi, Ive De Smet, Zhongfu Ni, Qixin Sun and Mingming Xin
J Integr Plant Biol 2023, 65 (12): 2587-2603.  
doi: 10.1111/jipb.13574
Abstract (Browse 81)  |   Save
Interploidy hybridization between hexaploid and tetraploid genotypes occurred repeatedly during genomic introgression events throughout wheat evolution, and is commonly employed in wheat breeding programs. Hexaploid wheat usually serves as maternal parent because the reciprocal cross generates progeny with severe defects and poor seed germination, but the underlying mechanism is poorly understood. Here, we performed detailed analysis of phenotypic variation in endosperm between two interploidy reciprocal crosses arising from tetraploid (Triticum durum, AABB) and hexaploid wheat (Triticum aestivum, AABBDD). In the paternal- versus the maternal-excess cross, the timing of endosperm cellularization was delayed and starch granule accumulation in the endosperm was repressed, causing reduced germination percentage. The expression profiles of genes involved in nutrient metabolism differed strongly between these endosperm types. Furthermore, expression patterns of parental alleles were dramatically disturbed in interploidy versus intraploidy crosses, leading to increased number of imprinted genes. The endosperm-specific TaLFL2 showed a paternally imprinted expression pattern in interploidy crosses partially due to allele-specific DNA methylation. Paternal TaLFL2 binds to and represses a nutrient accumulation regulator TaNAC019, leading to reduced storage protein and starch accumulation during endosperm development in paternal-excess cross, as confirmed by interploidy crosses between tetraploid wild-type and clustered regularly interspaced palindromic repeats (CRISPR)-CRISPR-associated protein 9 generated hexaploid mutants. These findings reveal a contribution of genomic imprinting to paternal-excess interploidy hybridization barriers during wheat evolution history and explains why experienced breeders preferentially exploit maternal-excess interploidy crosses in wheat breeding programs.
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Enhancing maize's nitrogen-fixing potential through ZmSBT3, a gene suppressing mucilage secretion
Jingyang Gao, Peijiang Feng, Jingli Zhang, Chaopei Dong, Zhao Wang, Mingxiang Chen, Zhongliang Yu, Bowen Zhao, Xin Hou, Huijuan Wang, Zhaokun Wu, Razia Sultana Jemim, Haidong Yu, Doudou Sun, Pei Jing, Jiafa Chen, Weibin Song, Xuecai Zhang, Zijian Zhou and Jianyu Wu,
J Integr Plant Biol 2023, 65 (12): 2645-2659.  
doi: 10.1111/jipb.13581
Abstract (Browse 125)  |   Save
Maize (Zea mays) requires substantial amounts of nitrogen, posing a challenge for its cultivation. Recent work discovered that some ancient Mexican maize landraces harbored diazotrophic bacteria in mucilage secreted by their aerial roots. To see if this trait is retained in modern maize, we conducted a field study of aerial root mucilage (ARM) in 258 inbred lines. We observed that ARM secretion is common in modern maize, but the amount significantly varies, and only a few lines have retained the nitrogen-fixing traits found in ancient landraces. The mucilage of the high-ARM inbred line HN5-724 had high nitrogen-fixing enzyme activity and abundant diazotrophic bacteria. Our genome-wide association study identified 17 candidate genes associated with ARM across three environments. Knockouts of one candidate gene, the subtilase family gene ZmSBT3, confirmed that it negatively regulates ARM secretion. Notably, the ZmSBT3 knockout lines had increased biomass and total nitrogen accumulation under nitrogen-free culture conditions. High ARM was associated with three ZmSBT3 haplotypes that were gradually lost during maize domestication, being retained in only a few modern inbred lines such as HN5-724. In summary, our results identify ZmSBT3 as a potential tool for enhancing ARM, and thus nitrogen fixation, in maize.
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SD-RLK28 positively regulates pollen hydration on stigmas as a PCP-Bβ receptor in Arabidopsis thaliana
Li Guo, Ziya Huang, Xingyu Chen, Min Yang, Miaomiao Yang, Ziwei Liu, Xuejie Han, Xiangjie Ma, Xiaoli Wang and Qiguo Gao
J Integr Plant Biol 2023, 65 (10): 2395-2406.  
doi: 10.1111/jipb.13547
Abstract (Browse 154)  |   Save
Pollen hydration on dry stigmas is strictly regulated by pollen–stigma interactions in Brassicaceae. Although several related molecular events have been described, the molecular mechanism underlying pollen hydration remains elusive. Multiple B-class pollen coat proteins (PCP-Bs) are involved in pollen hydration. Here, by analyzing the interactions of two PCP-Bs with three Arabidopsis thaliana stigmas strongly expressing S-domain receptor kinase (SD-RLK), we determined that SD-RLK28 directly interacts with PCP-Bβ. We investigated pollen hydration, pollen germination, pollen tube growth, and stigma receptivity in the sd-rlk28 and pcp-bβ mutants. PCP-Bβ acts in the pollen to regulate pollen hydration on stigmas. Loss of SD-RLK28 had no effect on pollen viability, and sd-rlk28 plants had normal life cycles without obvious defects. However, pollen hydration on sd-rlk28 stigmas was impaired and pollen tube growth in sd-rlk28 pistils was delayed. The defect in pollen hydration on sd-rlk28 stigmas was independent of changes in reactive oxygen species levels in stigmas. These results indicate that SD-RLK28 functions in the stigma as a PCP-Bβ receptor to positively regulate pollen hydration on dry stigmas.
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The aquaporin MePIP2;7 improves MeMGT9-mediated Mg2+ acquisition in cassava
Qiuxiang Ma, Yancai Feng, Shu Luo, Lu Cheng, Weijing Tong, Xinlu Lu, Youzhi Li and Peng Zhang
J Integr Plant Biol 2023, 65 (10): 2349-2367.  
doi: 10.1111/jipb.13552
Abstract (Browse 129)  |   Save
Aquaporins are important transmembrane water transport proteins which transport water and several neutral molecules. However, how aquaporins are involved in the synergistic transport of Mg2+ and water remains poorly understood. Here, we found that the cassava aquaporin MePIP2;7 was involved in Mg2+ transport through interaction with MeMGT9, a lower affinity magnesium transporter protein. Knockdown of MePIP2;7 in cassava led to magnesium deficiency in basal mature leaves with chlorosis and necrotic spots on their edges and starch over-accumulation. Mg2+ content was significantly decreased in leaves and roots of MePIP2;7-RNA interference (PIP-Ri) plants grown in both field and Mg2+-free hydroponic solution. Xenopus oocyte injection analysis verified that MePIP2;7 possessed the ability to transport water only and MeMGT9 was responsible for Mg2+ efflux. More importantly, MePIP2;7 improved the transportability of Mg2+ via MeMGT9 as verified using the CM66 mutant complementation assay and Xenopus oocytes expressing system. Yeast two-hybrid, bimolecular fluorescence complementation, co-localization, and co-immunoprecipitation assays demonstrated the direct protein–protein interaction between MePIP2;7 and MeMGT9 in vivo. Mg2+ flux was significantly elevated in MePIP2;7-overexpressing lines in hydroponic solution through non-invasive micro-test technique analysis. Under Mg2+-free condition, the retarded growth of PIP-Ri transgenic plants could be recovered with Mg2+ supplementation. Taken together, our results demonstrated the synergistic effect of the MePIP2;7 and MeMGT9 interaction in regulating water and Mg2+ absorption and transport in cassava.
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MaBEL1 regulates banana fruit ripening by activating cell wall and starch degradation-related genes
Zunyang Song, Xiaoyang Zhu, Xiuhua Lai, Hangcong Chen, Lihua Wang, Yulin Yao, Weixin Chen and Xueping Li
J Integr Plant Biol 2023, 65 (9): 2036-2055.  
doi: 10.1111/jipb.13506
Abstract (Browse 161)  |   Save
Banana is a typical subtropical fruit, sensitive to chilling injuries and prone to softening disorder. However, the underlying regulatory mechanisms of the softening disorder caused by cold stress remain obscure. Herein, we found that BEL1-LIKE HOMEODOMAIN transcription factor 1 (MaBEL1) and its associated proteins regulate the fruit softening and ripening process. The transcript and protein levels of MaBEL1 were up-regulated with fruit ripening but severely repressed by the chilling stress. Moreover, the MaBEL1 protein interacted directly with the promoters of the cell wall and starch degradation-related genes, such as MaAMY3, MaXYL32, and MaEXP-A8. The transient overexpression of MaBEL1 alleviated fruit chilling injury and ripening disorder caused by cold stress and promoted fruit softening and ripening of “Fenjiao” banana by inducing ethylene production and starch and cell wall degradation. The accelerated ripening was also validated by the ectopic overexpression in tomatoes. Conversely, MaBEL1-silencing aggravated the chilling injury and ripening disorder and repressed fruit softening and ripening by inhibiting ethylene production and starch and cell wall degradation. MaABI5-like and MaEBF1, the two positive regulators of the fruit softening process, interacted with MaBEL1 to enhance the promoter activity of the starch and cell wall degradation-related genes. Moreover, the F-box protein MaEBF1 does not modulate the degradation of MaBEL1, which regulates the transcription of MaABI5-like protein. Overall, we report a novel MaBEL1-MaEBF1-MaABI5-like complex system that mediates the fruit softening and ripening disorder in “Fenjiao” bananas caused by cold stress.
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Fusarium-produced vitamin B6 promotes the evasion of soybean resistance by Phytophthora sojae
Shuchen Wang, Xiaoyi Zhang, Zhichao Zhang, Yun Chen, Qing Tian, Dandan Zeng, Miao Xu, Yan Wang, Suomeng Dong, Zhonghua Ma, Yuanchao Wang, Xiaobo Zheng and Wenwu Ye
J Integr Plant Biol 2023, 65 (9): 2204-2217.  
doi: 10.1111/jipb.13505
Abstract (Browse 121)  |   Save
Plants can be infected by multiple pathogens concurrently in natural systems. However, pathogen–pathogen interactions have rarely been studied. In addition to the oomycete Phytophthora sojae, fungi such as Fusarium spp. also cause soybean root rot. In a 3-year field investigation, we discovered that P. sojae and Fusarium spp. frequently coexisted in diseased soybean roots. Out of 336 P. sojae–soybean–Fusarium combinations, more than 80% aggravated disease. Different Fusarium species all enhanced P. sojae infection when co-inoculated on soybean. Treatment with Fusarium secreted non-proteinaceous metabolites had an effect equal to the direct pathogen co-inoculation. By screening a Fusarium graminearum mutant library, we identified Fusarium promoting factor of Phytophthora sojae infection 1 (Fpp1), encoding a zinc alcohol dehydrogenase. Fpp1 is functionally conserved in Fusarium and contributes to metabolite-mediated infection promotion, in which vitamin B6 (VB6) produced by Fusarium is key. Transcriptional and functional analyses revealed that Fpp1 regulates two VB6 metabolism genes, and VB6 suppresses expression of soybean disease resistance-related genes. These results reveal that co-infection with Fusarium promotes loss of P. sojae resistance in soybean, information that will inform the sustainable use of disease-resistant crop varieties and provide new strategies to control soybean root rot.
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Rice pollen-specific OsRALF17 and OsRALF19 are essential for pollen tube growth
Eui‐Jung Kim, Ji‐Hyun Kim, Woo‐Jong Hong, Eun Young Kim, Myung‐Hee Kim, Su Kyoung Lee, Cheol Woo Min, Sun Tae Kim, Soon Ki Park, Ki‐Hong Jung and Yu‐Jin Kim
J Integr Plant Biol 2023, 65 (9): 2218-2236.  
doi: 10.1111/jipb.13508
Abstract (Browse 152)  |   Save
Pollen tube growth is essential for successful double fertilization, which is critical for grain yield in crop plants. Rapid alkalinization factors (RALFs) function as ligands for signal transduction during fertilization. However, functional studies on RALF in monocot plants are lacking. Herein, we functionally characterized two pollen-specific RALFs in rice (Oryza sativa) using multiple clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9-induced loss-of-function mutants, peptide treatment, expression analyses, and tag reporter lines. Among the 41 RALF members in rice, OsRALF17 was specifically expressed at the highest level in pollen and pollen tubes. Exogenously applied OsRALF17 or OsRALF19 peptide inhibited pollen tube germination and elongation at high concentrations but enhanced tube elongation at low concentrations, indicating growth regulation. Double mutants of OsRALF17 and OsRALF19 (ralf17/19) exhibited almost full male sterility with defects in pollen hydration, germination, and tube elongation, which was partially recovered by exogenous treatment with OsRALF17 peptide. This study revealed that two partially functionally redundant OsRALF17 and OsRALF19 bind to Oryza sativa male-gene transfer defective 2 (OsMTD2) and transmit reactive oxygen species signals for pollen tube germination and integrity maintenance in rice. Transcriptomic analysis confirmed their common downstream genes, in osmtd2 and ralf17/19. This study provides new insights into the role of RALF, expanding our knowledge of the biological role of RALF in regulating rice fertilization.
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Cell type-specific proteomics uncovers a RAF15-SnRK2.6/OST1 kinase cascade in guard cells
Hongliang Wang, Yubei Wang, Tian Sang, Zhen Lin, Rongxia Li, Weiwei Ren, Xin Shen, Bing Zhao, Xiao Wang, Xuebin Zhang, Shaoqun Zhou, Shaojun Dai, Honghong Hu, Chun‐Peng Song and Pengcheng Wang
J Integr Plant Biol 2023, 65 (9): 2122-2137.  
doi: 10.1111/jipb.13536
Abstract (Browse 178)  |   Save
Multicellular organisms such as plants contain various cell types with specialized functions. Analyzing the characteristics of each cell type reveals specific cell functions and enhances our understanding of organization and function at the organismal level. Guard cells (GCs) are specialized epidermal cells that regulate the movement of the stomata and gaseous exchange, and provide a model genetic system for analyzing cell fate, signaling, and function. Several proteomics analyses of GC are available, but these are limited in depth. Here we used enzymatic isolation and flow cytometry to enrich GC and mesophyll cell protoplasts and perform in-depth proteomics in these two major cell types in Arabidopsis leaves. We identified approximately 3,000 proteins not previously found in the GC proteome and more than 600 proteins that may be specific to GC. The depth of our proteomics enabled us to uncover a guard cell-specific kinase cascade whereby Raf15 and Snf1-related kinase2.6 (SnRK2.6)/OST1(open stomata 1) mediate abscisic acid (ABA)-induced stomatal closure. RAF15 directly phosphorylated SnRK2.6/OST1 at the conserved Ser175 residue in its activation loop and was sufficient to reactivate the inactive form of SnRK2.6/OST1. ABA-triggered SnRK2.6/OST1 activation and stomatal closure was impaired in raf15 mutants. We also showed enrichment of enzymes and flavone metabolism in GC, and consistent, dramatic accumulation of flavone metabolites. Our study answers the long-standing question of how ABA activates SnRK2.6/OST1 in GCs and represents a resource potentially providing further insights into the molecular basis of GC and mesophyll cell development, metabolism, structure, and function.
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Artificial evolution of OsEPSPS through an improved dual cytosine and adenine base editor generated a novel allele conferring rice glyphosate tolerance
Chen Zhang, Xue Zhong, Shaoya Li, Lei Yan, Jingying Li, Yubing He, Yong Lin, Yangjun Zhang and Lanqin Xia
J Integr Plant Biol 2023, 65 (9): 2194-2203.  
doi: 10.1111/jipb.13543
Abstract (Browse 178)  |   Save
Exploiting novel endogenous glyphosate-tolerant alleles is highly desirable and has promising potential for weed control in rice breeding. Here, through fusions of different effective cytosine and adenine deaminases with nCas9-NG, we engineered an effective surrogate two-component composite base editing system, STCBE-2, with improved C-to-T and A-to-G base editing efficiency and expanded the editing window. Furthermore, we targeted a rice endogenous OsEPSPS gene for artificial evolution through STCBE-2-mediated near-saturated mutagenesis. After hygromycin and glyphosate selection, we identified a novel OsEPSPS allele with an Asp-213-Asn (D213N) mutation (OsEPSPS-D213N) in the predicted glyphosate-binding domain, which conferred rice plants reliable glyphosate tolerance and had not been reported or applied in rice breeding. Collectively, we developed a novel dual base editor which will be valuable for artificial evolution of important genes in crops. And the novel glyphosate-tolerant rice germplasm generated in this study will benefit weeds management in rice paddy fields.
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The basic helix-loop-helix transcription factor gene, OsbHLH38, plays a key role in controlling rice salt tolerance
Fengping Du, Yinxiao Wang, Juan Wang, Yingbo Li, Yue Zhang, Xiuqin Zhao, Jianlong Xu, Zhikang Li, Tianyong Zhao, Wensheng Wang and Binying Fu
J Integr Plant Biol 2023, 65 (8): 1859-1873.  
doi: 10.1111/jipb.13489
Abstract (Browse 366)  |   Save
The plant hormone abscisic acid (ABA) is crucial for plant seed germination and abiotic stress tolerance. However, the association between ABA sensitivity and plant abiotic stress tolerance remains largely unknown. In this study, 436 rice accessions were assessed for their sensitivity to ABA during seed germination. The considerable diversity in ABA sensitivity among rice germplasm accessions was primarily reflected by the differentiation between the Xian (indica) and Geng (japonica) subspecies and between the upland-Geng and lowland-Geng ecotypes. The upland-Geng accessions were most sensitive to ABA. Genome-wide association analyses identified four major quantitative trait loci containing 21 candidate genes associated with ABA sensitivity of which a basic helix-loop-helix transcription factor gene, OsbHLH38, was the most important for ABA sensitivity. Comprehensive functional analyses using knockout and overexpression transgenic lines revealed that OsbHLH38 expression was responsive to multiple abiotic stresses. Overexpression of OsbHLH38 increased seedling salt tolerance, while knockout of OsbHLH38 increased sensitivity to salt stress. A salt-responsive transcription factor, OsDREB2A, interacted with OsbHLH38 and was directly regulated by OsbHLH38. Moreover, OsbHLH38 affected rice abiotic stress tolerance by mediating the expression of a large set of transporter genes of phytohormones, transcription factor genes, and many downstream genes with diverse functions, including photosynthesis, redox homeostasis, and abiotic stress responsiveness. These results demonstrated that OsbHLH38 is a key regulator in plant abiotic stress tolerance.
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Arabidopsis pollen-specific glycerophosphodiester phosphodiesterase-like genes are essential for pollen tube tip growth
Chong Wang, Hao Cheng, Wenjing Xu, Jingshi Xue, Xinguo Hua, Guimin Tong, Xujun Ma, Chuanping Yang, Xingguo Lan, Shi‐Yi Shen, Zhongnan Yang, Jirong Huang and Yuxiang Cheng
J Integr Plant Biol 2023, 65 (8): 2001-2017.  
doi: 10.1111/jipb.13490
Abstract (Browse 151)  |   Save
In angiosperms, pollen tube growth is critical for double fertilization and seed formation. Many of the factors involved in pollen tube tip growth are unknown. Here, we report the roles of pollen-specific GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE-LIKE (GDPD-LIKE) genes in pollen tube tip growth. Arabidopsis thaliana GDPD-LIKE6 (AtGDPDL6) and AtGDPDL7 were specifically expressed in mature pollen grains and pollen tubes and green fluorescent protein (GFP)-AtGDPDL6 and GFP-AtGDPDL7 fusion proteins were enriched at the plasma membrane at the apex of forming pollen tubes. Atgdpdl6 Atgdpdl7 double mutants displayed severe sterility that was rescued by genetic complementation with AtGDPDL6 or AtGDPDL7. This sterility was associated with defective male gametophytic transmission. Atgdpdl6 Atgdpdl7 pollen tubes burst immediately after initiation of pollen germination in vitro and in vivo, consistent with the thin and fragile walls in their tips. Cellulose deposition was greatly reduced along the mutant pollen tube tip walls, and the localization of pollen-specific CELLULOSE SYNTHASE-LIKE D1 (CSLD1) and CSLD4 was impaired to the apex of mutant pollen tubes. A rice pollen-specific GDPD-LIKE protein also contributed to pollen tube tip growth, suggesting that members of this family have conserved functions in angiosperms. Thus, pollen-specific GDPD-LIKEs mediate pollen tube tip growth, possibly by modulating cellulose deposition in pollen tube walls.
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GmJAZ3 interacts with GmRR18a and GmMYC2a to regulate seed traits in soybean
Yang Hu, Yue Liu, Jian‐Jun Tao, Long Lu, Zhi‐Hao Jiang, Jun‐Jie Wei, Chun‐Mei Wu, Cui‐Cui Yin, Wei Li, Ying‐Dong Bi, Yong‐Cai Lai, Wei Wei, Wan‐Ke Zhang, Shou‐Yi Chen and Jin‐Song Zhang
J Integr Plant Biol 2023, 65 (8): 1983-2000.  
doi: 10.1111/jipb.13494
Abstract (Browse 348)  |   Save
Seed weight is usually associated with seed size and is one of the important agronomic traits that determine yield. Understanding of seed weight control is limited, especially in soybean plants. Here we show that Glycine max JASMONATE-ZIM DOMAIN 3 (GmJAZ3), a gene identified through gene co-expression network analysis, regulates seed-related traits in soybean. Overexpression of GmJAZ3 promotes seed size/weight and other organ sizes in stable transgenic soybean plants likely by increasing cell proliferation. GmJAZ3 interacted with both G. max RESPONSE REGULATOR 18a (GmRR18a) and GmMYC2a to inhibit their transcriptional activation of cytokinin oxidase gene G. max CYTOKININ OXIDASE 3-4 (GmCKX3-4), which usually affects seed traits. Meanwhile, the GmRR18a binds to the promoter of GmMYC2a and activates GmMYC2a gene expression. In GmJAZ3-overexpressing soybean seeds, the protein contents were increased while the fatty acid contents were reduced compared to those in the control seeds, indicating that the GmJAZ3 affects seed size/weight and compositions. Natural variation in JAZ3 promoter region was further analyzed and Hap3 promoter correlates with higher promoter activity, higher gene expression and higher seed weight. The Hap3 promoter may be selected and fixed during soybean domestication. JAZ3 orthologs from other plants/crops may also control seed size and weight. Taken together, our study reveals a novel molecular module GmJAZ3-GmRR18a/GmMYC2a-GmCKXs for seed size and weight control, providing promising targets during soybean molecular breeding for better seed traits.
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Cited: Web of Science(3)
AtMAC stabilizes the phragmoplast by crosslinking microtubules and actin filaments during cytokinesis
Pingzhou Du, Yu Liu, Lu Deng, Dong Qian, Xiuhua Xue, Ting Yang, Tonghui Li, Yun Xiang and Haiyun Ren
J Integr Plant Biol 2023, 65 (8): 1950-1965.  
doi: 10.1111/jipb.13497
Abstract (Browse 109)  |   Save
The phragmoplast, a structure crucial for the completion of cytokinesis in plant cells, is composed of antiparallel microtubules (MTs) and actin filaments (AFs). However, how the parallel structure of phragmoplast MTs and AFs is maintained, especially during centrifugal phragmoplast expansion, remains elusive. Here, we analyzed a new Arabidopsis thaliana MT and AF crosslinking protein (AtMAC). When AtMAC was deleted, the phragmoplast showed disintegrity during centrifugal expansion, and the resulting phragmoplast fragmentation led to incomplete cell plates. Overexpression of AtMAC increased the resistance of phragmoplasts to depolymerization and caused the formation of additional phragmoplasts during cytokinesis. Biochemical experiments showed that AtMAC crosslinked MTs and AFs in vitro, and the truncated AtMAC protein, N-CC1, was the key domain controlling the ability of AtMAC. Further analysis showed that N-CC1(51–154) is the key domain for binding MTs, and N-CC1(51–125) for binding AFs. In conclusion, AtMAC is the novel MT and AF crosslinking protein found to be involved in regulation of phragmoplast organization during centrifugal phragmoplast expansion, which is required for complete cytokinesis.
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Cited: Web of Science(1)
NIGT1 represses plant growth and mitigates phosphate starvation signaling to balance the growth response tradeoff in rice
Yuxin Zhang, Qianqian Zhang, Meina Guo, Xueqing Wang, Tianjie Li, Qingyu Wu, Lihui Li, Keke Yi and Wenyuan Ruan
J Integr Plant Biol 2023, 65 (8): 1874-1889.  
doi: 10.1111/jipb.13496
Abstract (Browse 171)  |   Save
Inorganic phosphate (Pi) availability is an important factor which affects the growth and yield of crops, thus an appropriate and effective response to Pi fluctuation is critical. However, how crops orchestrate Pi signaling and growth under Pi starvation conditions to optimize the growth defense tradeoff remains unclear. Here we show that a Pi starvation-induced transcription factor NIGT1 (NITRATE-INDUCIBLE GARP-TYPE TRANSCRIPTIONAL REPRESSOR 1) controls plant growth and prevents a hyper-response to Pi starvation by directly repressing the expression of growth-related and Pi-signaling genes to achieve a balance between growth and response under a varying Pi environment. NIGT1 directly binds to the promoters of Pi starvation signaling marker genes, like IPS1, miR827, and SPX2, under Pi-deficient conditions to mitigate the Pi-starvation responsive (PSR). It also directly represses the expression of vacuolar Pi efflux transporter genes VPE1/2 to regulate plant Pi homeostasis. We further demonstrate that NIGT1 constrains shoot growth by repressing the expression of growth-related regulatory genes, including brassinolide signal transduction master regulator BZR1, cell division regulator CYCB1;1, and DNA replication regulator PSF3. Our findings reveal the function of NIGT1 in orchestrating plant growth and Pi starvation signaling, and also provide evidence that NIGT1 acts as a safeguard to avoid hyper-response during Pi starvation stress in rice.
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DIW1 encoding a clade I PP2C phosphatase negatively regulates drought tolerance by de-phosphorylating TaSnRK1.1 in wheat
Jingyi Wang, Chaonan Li, Long Li, Lifeng Gao, Ge Hu, Yanfei Zhang, Matthew P. Reynolds, Xueyong Zhang, Jizeng Jia, Xinguo Mao and Ruilian Jing
J Integr Plant Biol 2023, 65 (8): 1918-1936.  
doi: 10.1111/jipb.13504
Abstract (Browse 222)  |   Save
Drought seriously impacts wheat production (Triticum aestivum L.), while the exploitation and utilization of genes for drought tolerance are insufficient. Leaf wilting is a direct reflection of drought tolerance in plants. Clade A PP2Cs are abscisic acid (ABA) co-receptors playing vital roles in the ABA signaling pathway, regulating drought response. However, the roles of other clade PP2Cs in drought tolerance, especially in wheat, remain largely unknown. Here, we identified a gain-of-function drought-induced wilting 1 (DIW1) gene from the wheat Aikang 58 mutant library by map-based cloning, which encodes a clade I protein phosphatase 2C (TaPP2C158) with enhanced protein phosphatase activity. Phenotypic analysis of overexpression and CRISPR/Cas9 mutant lines demonstrated that DIW1/TaPP2C158 is a negative regulator responsible for drought resistance. We found that TaPP2C158 directly interacts with TaSnRK1.1 and de-phosphorylates it, thus inactivating the TaSnRK1.1–TaAREB3 pathway. TaPP2C158 protein phosphatase activity is negatively correlated with ABA signaling. Association analysis suggested that C-terminal variation of TaPP2C158 changing protein phosphatase activity is highly correlated with the canopy temperature, and seedling survival rate under drought stress. Our data suggest that the favorable allele with lower phosphatase activity of TaPP2C158 has been positively selected in Chinese breeding history. This work benefits us in understanding the molecular mechanism of wheat drought tolerance, and provides elite genetic resources and molecular markers for improving wheat drought tolerance.
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Zinc-finger protein GmZF351 improves both salt and drought stress tolerance in soybean
Wei Wei, Long Lu, Xiao‐Hua Bian, Qing‐Tian Li, Jia‐Qi Han, Jian‐Jun Tao, Cui‐Cui Yin, Yong‐Cai Lai, Wei Li, Ying‐Dong Bi, Wei‐Qun Man, Shou‐Yi Chen, Jin‐Song Zhang and Wan‐Ke Zhang
J Integr Plant Biol 2023, 65 (7): 1636-1650.  
doi: 10.1111/jipb.13474
Abstract (Browse 178)  |   Save
Abiotic stress is one of the most important factors reducing soybean yield. It is essential to identify regulatory factors contributing to stress responses. A previous study found that the tandem CCCH zinc-finger protein GmZF351 is an oil level regulator. In this study, we discovered that the GmZF351 gene is induced by stress and that the overexpression of GmZF351 confers stress tolerance to transgenic soybean. GmZF351 directly regulates the expression of GmCIPK9 and GmSnRK, leading to stomata closing, by binding to their promoter regions, which carry two CT(G/C)(T/A)AA elements. Stress induction of GmZF351 is mediated through reduction in the H3K27me3 level at the GmZF351 locus. Two JMJ30-demethylase-like genes, GmJMJ30-1 and GmJMJ30-2, are involved in this demethylation process. Overexpression of GmJMJ30-1/2 in transgenic hairy roots enhances GmZF351 expression mediated by histone demethylation and confers stress tolerance to soybean. Yield-related agronomic traits were evaluated in stable GmZF351-transgenic plants under mild drought stress conditions. Our study reveals a new mode of GmJMJ30-GmZF351 action in stress tolerance, in addition to that of GmZF351 in oil accumulation. Manipulation of the components in this pathway is expected to improve soybean traits and adaptation under unfavorable environments.
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Cited: Web of Science(4)
Differential SW16.1 allelic effects and genetic backgrounds contributed to increased seed weight after soybean domestication
Xianlian Chen, Cheng Liu, Pengfei Guo, Xiaoshuai Hao, Yongpeng Pan, Kai Zhang, Wusheng Liu, Lizhi Zhao, Wei Luo, Jianbo He, Yanzhu Su, Ting Jin, Fenfen Jiang, Si Wang, Fangdong Liu, Rongzhou Xie, Changgen Zhen, Wei Han, Guangnan Xing, Wubin Wang, Shancen Zhao, Yan Li and Junyi Gai
J Integr Plant Biol 2023, 65 (7): 1734-1752.  
doi: 10.1111/jipb.13480
Abstract (Browse 188)  |   Save
Although seed weight has increased following domestication from wild soybean (Glycine soja) to cultivated soybean (Glycine max), the genetic basis underlying this change is unclear. Using mapping populations derived from chromosome segment substitution lines of wild soybean, we identified SW16.1 as the causative gene underlying a major quantitative trait locus controlling seed weight. SW16.1 encodes a nucleus-localized LIM domain-containing protein. Importantly, the GsSW16.1 allele from wild soybean accession N24852 had a negative effect on seed weight, whereas the GmSW16.1 allele from cultivar NN1138-2 had a positive effect. Gene expression network analysis, reverse-transcription quantitative polymerase chain reaction, and promoter-luciferase reporter transient expression assays suggested that SW16.1 regulates the transcription of MT4, a positive regulator of seed weight. The natural variations in SW16.1 and other known seed weight genes were analyzed in soybean germplasm. The SW16.1 polymorphism was associated with seed weight in 247 soybean accessions, showing much higher frequency of positive-effect alleles in cultivated soybean than in wild soybean. Interestingly, gene allele matrix analysis of the known seed weight genes revealed that G. max has lost 38.5% of the G. soja alleles and that most of the lost alleles had negative effects on seed weight. Our results suggest that eliminating negative alleles from G. soja led to a higher frequency of positive alleles and changed genetic backgrounds in G. max, which contributed to larger seeds in cultivated soybean after domestication from wild soybean. Our findings provide new insights regarding soybean domestication and should assist current soybean breeding programs.
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Cited: Web of Science(1)
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