Studies on Highly Efficient Planting of Transgenic Cotton

Abstract

Abstract: Foreign insecticidal genes were transferred into 4 upland cotton ( Gossypium hirsutum L. ) cultivars by Agrobacterium-mediated transformation. It was found that planting style has a critical influence on the survival rate of the transgenic plantlets. Direct transplanting, culturing in liquid medium or vermiculite before transplanting were not proper for planting transgenic plantlets. The survival rate was quite low and hardening time was rather long. Graft could greatly enhance the survival rate and efficiently reduced the hardening time, thus paves the way for cotton improvements through genetic engineering and other biotechnology.

Key words: Gossypium hirsuturn, Transformation, Transplant, Graft

WANG Wei, CHEN Wan-Xin, ZHU Zhen, XU Hong-Lin, GAO Yue-Feng, WU Qian, ZHU Yu, GUO Zhong-Chen, LI Xiang-Hui. Studies on Highly Efficient Planting of Transgenic Cotton[J]. J Integr Plant Biol., 1999, 41(10): -.

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[7]	Martin A. J. Parry, and Malcolm J. Hawkesford. An Integrated Approach to Crop Genetic Improvement [J]. J Integr Plant Biol., 2012, 54(4): 250-259.
[8]	Michitaka Notaguchi, Shmuel Wolf, and William J. Lucas. Phloem-Mobile Aux/IAA Transcripts Target to the Root Tip and Modify Root Architecture [J]. J Integr Plant Biol., 2012, 54(10): 760-772.
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Studies on Highly Efficient Planting of Transgenic Cotton

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