J Integr Plant Biol. ›› 2006, Vol. 48 ›› Issue (10): -.DOI: 10.1111/j.1744-7909.2006.00305.x

• Research Articles •    

Identification of Novel Stress-responsive Transcription Factor Genes in Rice by cDNA Array Analysis

Cong-Qing Wu, Hong-Hong Hu, Ya Zeng, Da-Cheng Liang, Ka-Bin Xie,Jian-Wei Zhang, Zhao-Hui Chu and Li-Zhong Xiong*   

Abstract: Numerous studies have shown that array of transcription factors has a role in regulating plant responses to environmental stresses. Only a small portion of them however, have been identified or characterized. More than 2 300 putative transcription factors were predicted in the rice genome and more than half of them were supported by expressed sequences. With an attempt to identify novel transcription factors involved in the stress responses, a cDNA array containing 753 putative rice transcription factors was generated to analyze the transcript profiles of these genes under drought and salinity stresses and abscisic acid treatment at seedling stage of rice. About 80% of these transcription factors showed detectable levels of transcript in seedling leaves. A total of 18 up-regulated transcription factors and 29 down-regulated transcription factors were detected with the folds of changes from 2.0 to 20.5 in at least one stress treatment. Most of these stress-responsive genes have not been reported and the expression patterns for five genes under stress conditions were further analyzed by RNA gel blot analysis. These novel stress-responsive transcription factors provide new opportunities to study the regulation of gene expression in plants under stress conditions.(Author for correspondence. Tel: +86 (0)27 8728 1536; Fax: +86 (0)27 8728 7092; E-mail: lizhongx@mail.hzau.edu.cn)

Key words: abiotic stress cDNA array, Oryza sativa, transcription factor.

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q