J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (3): 225-234.DOI: 10.1111/j.1744-7909.2008.00766.x

Special Issue: Plant Signal Transduction

• Cell and Developmental Biology •     Next Articles

Involvement of Polyamine Oxidase in Abscisic Acid-induced Cytosolic Antioxidant Defense in Leaves of Maize

Beibei Xue1, Aying Zhang1 and Mingyi Jiang1*   

  1. 1College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2008-02-27 Accepted:2008-06-18 Published:2008-11-12
  • About author: *Author for correspondence Tel: +86 25 8439 6372; Fax: +86 25 8439 6542; E-mail: myjiang@njau.edu.cn
  • Supported by:
    Supported by the Science Foundation for New Teachers of Doctoral Subject Point of the Ministry of Education of China (20070307018).

Abstract:

Using pharmacological and biochemical approaches, the role of maize polyamine oxidase (MPAO) in abscisic acid (ABA)-induced antioxidant defense in leaves of maize (Zea mays L.) plants was investigated. Exogenous ABA treatment enhanced the expression of the MPAO gene and the activities of apoplastic MPAO. Pretreatment with two different inhibitors for apoplastic MPAO partly reduced hydrogen peroxide (H2O2) accumulation induced by ABA and blocked the ABA-induced expression of the antioxidant genes superoxide dismutase 4 and cytosolic ascorbate peroxidase and the activities of the cytosolic antioxidant enzymes. Treatment with spermidine, the optimum substrate of MPAO, also induced the expression and the activities of the antioxidant enzymes, and the upregulation of the antioxidant enzymes was prevented by two inhibitors of MPAO and two scavengers of H2O2. These results suggest that MPAO contributes to ABA-induced cytosolic antioxidant defense through H2O2, a Spd catabolic product. Using pharmacological and biochemical approaches, the role of maize polyamine oxidase (MPAO) in abscisic acid (ABA)-induced antioxidant defense in leaves of maize (Zea mays L.) plants was investigated. Exogenous ABA treatment enhanced the expression of the MPAO gene and the activities of apoplastic MPAO. Pretreatment with two different inhibitors for apoplastic MPAO partly reduced hydrogen peroxide (H2O2) accumulation induced by ABA and blocked the ABA-induced expression of the antioxidant genes superoxide dismutase 4 and cytosolic ascorbate peroxidase and the activities of the cytosolic antioxidant enzymes. Treatment with spermidine, the optimum substrate of MPAO, also induced the expression and the activities of the antioxidant enzymes, and the upregulation of the antioxidant enzymes was prevented by two inhibitors of MPAO and two scavengers of H2O2. These results suggest that MPAO contributes to ABA-induced cytosolic antioxidant defense through H2O2, a Spd catabolic product.

Xue B, Zhang A, Jiang M (2009) Involvement of polyamine oxidase in abscisic acid-induced cytosolic antioxidant defense in leaves of maize. J. Integr. Plant Biol. 51, 225–234.

Key words: abscisic acid, cytosolic antioxidant defense, hydrogen peroxide, polyamine oxidase, Zea mays

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