J Integr Plant Biol ›› 2015, Vol. 57 ›› Issue (6): 591-601.DOI: 10.1111/jipb.12296

Special Issue: Plant Signal Transduction

• Plant-environmental Interactions • Previous Articles    

Selective silencing of 2Cys and type-IIB Peroxiredoxins discloses their roles in cell redox state and stress signaling

Patrícia Vidigal1, Ana Montserrat Martin-Hernandez2, Cèlia Guiu-Aragonés2, Sara Amâncio1 and Luísa Carvalho1*   

  1. 1DRAT/CBAA, Instituto Superior de Agronomia, Universidade de Lisboa. Tapada da Ajuda, Lisboa, Portugal
    2IRTA, Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB) Campus UAB - Edificio CRAG, Bellaterra - Cerdanyola del Vallès, Barcelona
  • Received:2014-09-15 Accepted:2014-10-08 Published:2014-10-16
  • About author:*Correspondence: E-mail: lcarvalho@isa.ulisboa.pt

Abstract:

Peroxiredoxins (Prx) catalyse the reduction of hydrogen peroxide (H2O2) and, in association with catalases and other peroxidases, may participate in signal transduction by regulating intercellular H2O2 concentration that in turn can control gene transcription and cell signaling. Using virus-induced-gene-silencing (VIGS), 2-Cys Peroxiredoxin (2CysPrx) family and type-II Peroxiredoxin B (PrxIIB) gene were silenced in Nicotiana benthamiana, to study the impact that the loss of function of each Prx would have in the antioxidant system under control (22 °C) and severe heat stress conditions (48 °C). The results showed that both Prxs, although in different organelles, influence the regeneration of ascorbate to a significant extent, but with different purposes. 2CysPrx affects abscisic acid (ABA) biosynthesis through ascorbate, while PrxIIB does it probably through the xanthophyll cycle. Moreover, 2CysPrx is key in H2O2 scavenging and in consequence in the regulation of ABA signaling downstream of reactive oxygen species and PrxIIB provides an important assistance for H2O2 peroxisome scavenges.

 

Vidigal P, Martin-Hernandez AM, Guiu-Aragonés C, Amâncio S, Carvalho L (2015) Selective silencing of 2Cys and type-IIB Peroxiredoxins discloses their roles in cell redox state and stress signaling. J Integr Plant Biol 57: 591–601. doi: 10.1111/jipb.12296

Through the silencing of two different peroxiredoxin, it was possible to learn that 2Cys-Peroxiredoxin influences the biosynthesis of the stress hormone abscisic acid through the regeneration of ascorbate. Ascorbate regeneration is also affected by Peroxiredoxin-IIB through the xanthophyll cycle, another adaptive mechanism triggered in stressful conditions.

Key words: 2-Cys Peroxiredoxin, abscisic acid, ascorbate, heat stress, hydrogen peroxide, peroxisome, type-II Peroxiredoxin B

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