Selective silencing of 2Cys and type-IIB Peroxiredoxins discloses their roles in cell redox state and stress signaling

doi:10.1111/jipb.12296

J Integr Plant Biol ›› 2015, Vol. 57 ›› Issue (6): 591-601.DOI: 10.1111/jipb.12296

Special Issue: Plant Signal Transduction

• Plant-environmental Interactions • Previous Articles

Selective silencing of 2Cys and type-IIB Peroxiredoxins discloses their roles in cell redox state and stress signaling

Patrícia Vidigal¹, Ana Montserrat Martin-Hernandez², Cèlia Guiu-Aragonés², Sara Amâncio¹ and Luísa Carvalho^1*

¹DRAT/CBAA, Instituto Superior de Agronomia, Universidade de Lisboa. Tapada da Ajuda, Lisboa, Portugal
²IRTA, Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB) Campus UAB - Edificio CRAG, Bellaterra - Cerdanyola del Vallès, Barcelona

Received:2014-09-15 Accepted:2014-10-08 Published:2014-10-16
About author:^*Correspondence: E-mail: lcarvalho@isa.ulisboa.pt

Abstract

Abstract:

Peroxiredoxins (Prx) catalyse the reduction of hydrogen peroxide (H₂O₂) and, in association with catalases and other peroxidases, may participate in signal transduction by regulating intercellular H₂O₂ concentration that in turn can control gene transcription and cell signaling. Using virus-induced-gene-silencing (VIGS), 2-Cys Peroxiredoxin (2CysPrx) family and type-II Peroxiredoxin B (PrxIIB) gene were silenced in Nicotiana benthamiana, to study the impact that the loss of function of each Prx would have in the antioxidant system under control (22 °C) and severe heat stress conditions (48 °C). The results showed that both Prxs, although in different organelles, influence the regeneration of ascorbate to a significant extent, but with different purposes. 2CysPrx affects abscisic acid (ABA) biosynthesis through ascorbate, while PrxIIB does it probably through the xanthophyll cycle. Moreover, 2CysPrx is key in H₂O₂ scavenging and in consequence in the regulation of ABA signaling downstream of reactive oxygen species and PrxIIB provides an important assistance for H₂O₂ peroxisome scavenges.

Vidigal P, Martin-Hernandez AM, Guiu-Aragonés C, Amâncio S, Carvalho L (2015) Selective silencing of 2Cys and type-IIB Peroxiredoxins discloses their roles in cell redox state and stress signaling. J Integr Plant Biol 57: 591–601. doi: 10.1111/jipb.12296

Through the silencing of two different peroxiredoxin, it was possible to learn that 2Cys-Peroxiredoxin influences the biosynthesis of the stress hormone abscisic acid through the regeneration of ascorbate. Ascorbate regeneration is also affected by Peroxiredoxin-IIB through the xanthophyll cycle, another adaptive mechanism triggered in stressful conditions.

Key words: 2-Cys Peroxiredoxin, abscisic acid, ascorbate, heat stress, hydrogen peroxide, peroxisome, type-II Peroxiredoxin B

Patrícia Vidigal, Ana Montserrat Martin-Hernandez, Cèlia Guiu-Aragonés, Sara Amâncio, and Luísa Carvalho. Selective silencing of 2Cys and type-IIB Peroxiredoxins discloses their roles in cell redox state and stress signaling[J]. J Integr Plant Biol, 2015, 57(6): 591-601.

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Selective silencing of 2Cys and type-IIB Peroxiredoxins discloses their roles in cell redox state and stress signaling

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