J Integr Plant Biol. ›› 2015, Vol. 57 ›› Issue (3): 313-324.DOI: 10.1111/jipb.12246

Special Issue: Abiotic Stresses

• Plant-environmental Interactions • Previous Articles    

The CONSTANS-like 4 transcription factor, AtCOL4, positively regulates abiotic stress tolerance through an abscisic acid-dependent manner in Arabidopsis

Ji-Hee Min1, Jung-Sung Chung2, Kyeong-Hwan Lee3 and Cheol Soo Kim1*   

  1. 1Department of Plant Biotechnology, Chonnam National University, Gwangju 500-757, South Korea
    2Department of Agronomy, Gyeongsang National University, Jinju 660-701, South Korea
    3Department of Rural and Biosystems Engineering, Chonnam National University, Gwangju 500-757, South Korea
  • Received:2014-04-05 Accepted:2014-07-28 Published:2014-07-29
  • About author:*Correspondence: E-mail: cskim626@chonnam.ac.kr

Abstract:

The precise roles of the B-box zinc finger family of transcription factors in plant stress are poorly understood. Functional analysis was performed on AtCOL4, an Arabidopsis thaliana L. CONSTANS-like 4 protein that is a putative novel transcription factor, and which contains a predicted transcriptional activation domain. Analyses of an AtCOL4 promoter-β-glucuronidase (GUS) construct revealed substantial GUS activity in whole seedlings. The expression of AtCOL4 was strongly induced by abscisic acid (ABA), salt, and osmotic stress. Mutation in atcol4 resulted in increased sensitivity to ABA and salt stress during seed germination and the cotyledon greening process. In contrast, AtCOL4-overexpressing plants were less sensitive to ABA and salt stress compared to the wild type. Interestingly, in the presence of ABA or salt stress, the transcript levels of other ABA biosynthesis and stress-related genes were enhanced induction in AtCOL4-overexpressing and WT plants, rather than in the atcol4 mutant. Thus, AtCOL4 is involved in ABA and salt stress response through the ABA-dependent signaling pathway. Taken together, these findings provide compelling evidence that AtCOL4 is an important regulator for plant tolerance to abiotic stress.

 

Min JH, Chung JS, Lee KH, Kim CS (2015) The CONSTANS‐like 4 transcription factor, AtCOL4, positively regulates abiotic stress tolerance through an abscisic acid‐dependent manner in Arabidopsis. J Integr Plant Biol 57: 313–324. doi: 10.1111/jipb.12246

Key words: Abscisic acid, B-box zinc finger family, transcription factor, salt stress

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