J Integr Plant Biol. ›› 2013, Vol. 55 ›› Issue (11): 1054-1068.DOI: 10.1111/jipb.12104

Special Issue: Abiotic Stresses

• Molecular Physiology • Previous Articles     Next Articles

Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress

Lei Zhao1,2†, Mei Chen1†, Dongmei Cheng1,2, Haomeng Yang1, Yongle Sun1,2, Heyi Zhou1,2 and Fang Huang1*   

  1. 1Key Laboratory of Photobiology, Institute of Botany, the Chinese Academy of Sciences, Beijing, China
    2University of Chinese Academy of Sciences, Beijing, China
  • Received:2013-04-27 Accepted:2013-08-28 Published:2013-10-28
  • About author:These authors contributed equally to this work.
    *Corresponding author Tel: +86 10 6283 6692; Fax: +86 10 6259 4363; E-mail: fhuang@ibcas.ac.cn

Abstract:

The genome of unicellular green alga Chlamydomonas reinhardtii contains four genes encoding B-type methionine sulfoxide reductases, MSRB1.1, MSRB1.2, MSRB2.1, and MSRB2.2, with functions largely unknown. To understand the cell defense system mediated by the methionine sulfoxide reductases in Chlamydomonas, we analyzed expression and physiological roles of the MSRBs under different abiotic stress conditions using immunoblotting and quantitative polymerase chain reaction (PCR) analyses. We showed that the MSRB2.2 protein was accumulated in cells treated with high light (1,300 µE/m2 per s), whereas MSRB1.1 was accumulated in the cells under 1 mmol/L H2O2 treatment or sulfur depletion. We observed that the cells with the MSRB2.2 knockdown and overexpression displayed increased and decreased sensitivity to high light, respectively, based on in situ chlorophyll a fluorescence measures. We also observed that the cells with the MSRB1.1 knockdown and overexpression displayed decreased and increased tolerance to sulfur-depletion and oxidative stresses, respectively, based on growth and H2-producing performance. The physiological implications revealed from the experimental data highlight the importance of MSRB2.2 and MSRB1.1 in protecting Chlamydomonas cells against adverse conditions such as high-light, sulfur-depletion, and oxidative stresses.

Zhao L, Chen M, Cheng D, Yang H, Sun Y, Zhou H, Huang F (2013) Different B‐type methionine sulfoxide reductases in Chlamydomonas may protect the alga against high‐light, sulfur‐depletion, or oxidative stress. J. Integr. Plant Biol. 55(11), 1054–1068.

Key words: Chlamydomonas, high light, MSRB, oxidative stress, sulfur deprivation

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