J Integr Plant Biol ›› 2012, Vol. 54 ›› Issue (9): 652-662.DOI: 10.1111/j.1744-7909.2012.01150.x

Special Issue: Rice Genomics and Agriculture

• Plant-environmental Interactions • Previous Articles     Next Articles

Overexpression of Rice Sphingosine-1-Phoshpate Lyase Gene OsSPL1 in Transgenic Tobacco Reduces Salt and Oxidative Stress Tolerance

Huijuan Zhang, Jing Zhai, Jibo Mo, Dayong Li and Fengming Song*   

  1. State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310058, China
  • Received:2012-05-01 Accepted:2012-08-05 Published:2012-09-13
  • About author:*Corresponding author Tel: +86 0571 8898 2481; Fax: +86 0571 8898 2271; E-mail: fmsong@zju.edu.cn

Abstract:

Sphingolipids, including sphingosine-1-phosphate (S1P), have been shown to function as signaling mediators to regulate diverse aspects of plant growth, development, and stress response. In this study, we performed functional analysis of a rice (Oryza sativa) S1P lyase gene OsSPL1 in transgenic tobacco plants and explored its possible involvement in abiotic stress response. Overexpression of OsSPL1 in transgenic tobacco resulted in enhanced sensitivity to exogenous abscisic acid (ABA), and decreased tolerance to salt and oxidative stress, when compared with the wild type. Furthermore, the expression levels of some selected stress-related genes in OsSPL1-overexpressing plants were reduced after application of salt or oxidative stress, indicating that the altered responsiveness of stress-related genes may be responsible for the reduced tolerance in OsSPL1-overexpressing tobacco plants under salt and oxidative stress. Our results suggest that rice OsSPL1 plays an important role in abiotic stress responses.

Zhang H, Zhai J, Mo J, Li D, Song F (2012) Overexpression of rice sphingosine-1-phoshpate lyase gene OsSPL1 in transgenic tobacco reduces salt and oxidative stress tolerance. J. Integr. Plant Biol. 54(9), 652–662.

Key words: Sphingolipids, sphingosine-1-phosphate lyase (SPL), OsSPL1, salt stress, oxidative stress

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