J Integr Plant Biol. ›› 2016, Vol. 58 ›› Issue (12): 943-946.DOI: 10.1111/jipb.12502

• Letter to the Editor •     Next Articles

Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H2 evolution

Mei Chen1†, Jin Zhang1,2†, Lei Zhao1, Jiale Xing1,2, Lianwei Peng1, Tingyun Kuang1, Jean-David Rochaix3 and Fang Huang1*   

  1. 1Key Laboratory of Photobiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China
    2University of Chinese Academy of Sciences, Beijing 100049, China
    3Departments of Molecular Biology and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
  • Received:2016-09-23 Accepted:2016-10-14 Published:2016-10-20
  • About author:These authors contributed equally to this work.
    *Correspondence: E-mail: fhuang@ibcas.ac.cn

Abstract:

We have identified hpm91, a Chlamydomonas mutant lacking Proton Gradient Regulation5 (PGR5) capable of producing hydrogen (H2) for 25 days with more than 30-fold yield increase compared to wild type. Thus, hpm91 displays a higher capacity of H2 production than a previously characterized pgr5 mutant. Physiological and biochemical characterization of hpm91 reveal that the prolonged H2 production is due to enhanced stability of PSII, which correlates with increased reactive oxygen species (ROS) scavenging capacity during sulfur deprivation. This anti-ROS response appears to protect the photosynthetic electron transport chain from photo-oxidative damage and thereby ensures electron supply to the hydrogenase.

Key words: Chlamydomonas reinhardtii, hpm91, hydrogen photoproduction, oxidative stress, photosystem II, sulfur deprivation

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