The E3 ubiquitin ligase SINA1 and the protein kinase BIN2 cooperatively regulate PHR1 in apple anthocyanin biosynthesis

doi:10.1111/jipb.13538

J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (9): 2175-2193.DOI: 10.1111/jipb.13538

• Molecular Physiology • Previous Articles Next Articles

The E3 ubiquitin ligase SINA1 and the protein kinase BIN2 cooperatively regulate PHR1 in apple anthocyanin biosynthesis

Jian‐Ping An^1,2*, Hong‐Liang Li², Zhi‐Ying Liu², Da‐Ru Wang², Chun‐Xiang You^2* and Yuepeng Han^1*

1. CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Hubei Hongshan Laboratory, The Innovative Academy of Seed Design of Chinese Academy of Sciences, Wuhan 430074, China;
2. College of Horticulture Science and Engineering, Shandong Agricultural University, Tai‐An 271018, China
^*Correspondences: Jian‐Ping An (anjianping@sdau.edu.cn, Dr. An is fully responsible for the distributions of all materials associated with this article); Chun‐Xiang You (youchunxiang@sdau.edu.cn); Yuepeng Han (yphan@wbgcas.cn)

Received:2023-01-19 Accepted:2023-06-02 Online:2023-06-05 Published:2023-09-01

Abstract

Abstract: PHR1 (PHOSPHATE STARVATION RESPONSE1) plays key roles in the inorganic phosphate (Pi) starvation response and in Pi deficiency-induced anthocyanin biosynthesis in plants. However, the post-translational regulation of PHR1 is unclear, and the molecular basis of PHR1-mediated anthocyanin biosynthesis remains elusive. In this study, we determined that MdPHR1 was essential for Pi deficiency-induced anthocyanin accumulation in apple (Malus×domestica). MdPHR1 interacted with MdWRKY75, a positive regulator of anthocyanin biosynthesis, to enhance the MdWRKY75-activated transcription of MdMYB1, leading to anthocyanin accumulation. In addition, the E3 ubiquitin ligase SEVEN IN ABSENTIA1 (MdSINA1) negatively regulated MdPHR1-promoted anthocyanin biosynthesis via the ubiquitination-mediated degradation of MdPHR1. Moreover, the protein kinase apple BRASSINOSTEROID INSENSITIVE2 (MdBIN2) phosphorylated MdPHR1 and positively regulated MdPHR1-mediated anthocyanin accumulation by attenuating the MdSINA1-mediated ubiquitination degradation of MdPHR1. Taken together, these findings not only demonstrate the regulatory role of MdPHR1 in Pi starvation induced anthocyanin accumulation, but also provide an insight into the post-translational regulation of PHR1.

Key words: anthocyanin biosynthesis, apple, phosphate starvation response, phosphorylation, PHR1, ubiquitination

Jian‐Ping An, Hong‐Liang Li, Zhi‐Ying Liu, Da‐Ru Wang, Chun‐Xiang You and Yuepeng Han. The E3 ubiquitin ligase SINA1 and the protein kinase BIN2 cooperatively regulate PHR1 in apple anthocyanin biosynthesis[J]. J Integr Plant Biol., 2023, 65(9): 2175-2193.

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The E3 ubiquitin ligase SINA1 and the protein kinase BIN2 cooperatively regulate PHR1 in apple anthocyanin biosynthesis

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