J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (7): 654-662.DOI: 10.1111/j.1744-7909.2009.00844.x

Special Issue: Abiotic Stresses Plant Signal Transduction

• Plant-environmental Interactions • Previous Articles     Next Articles

Involvement of Protein Phosphorylation in Water Stress-induced Antioxidant Defense in Maize Leaves

Shucheng Xu1,2, Haidong Ding1, Fengxia Su1, Aying Zhang1 and Mingyi Jiang1,3*   

  1. 1 College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
    2 College of Life Sciences, Fuyang Teachers College, Fuyang 236032, China
    3 National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2009-01-01 Accepted:2009-04-16 Published:2009-06-25
  • About author: *Author for correspondence Tel: +86 25 8439 6372; Fax: +86 25 8439 6542; E-mail: myjiang@njau.edu.cn

Abstract:

Using pharmacological and biochemical approaches, the role of protein phosphorylation and the interrelationship between water stress-enhanced kinase activity, antioxidant enzyme activity, hydrogen peroxide(H2O2) accumulation and endogenous abscisic acid in maize (Zea mays L.) leaves were investigated. Water-stress upregulated the activities of total protein phosphorylation and Ca2+-dependent protein kinase, and the upregulation was blocked in abscisic acid-deficient vp5 mutant. Furthermore, pretreatments with a nicotinamide adenine dinucleotide phosphate oxidase inhibitor and a scavenger of H2O2 significantly reduced the increased activities of total protein kinase and Ca2+-dependent protein kinase in maize leaves exposed to water stress. Pretreatments with different protein kinase inhibitors also reduced the water stress-induced H2O2 production and the water stress-enhanced activities of antioxidant enzymes such as superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase. The data suggest that protein phosphorylation and H2O2 generation are required for water stress-induced antioxidant defense in maize leaves and that crosstalk between protein phosphorylation and H2O2 generation may occur.
 

Xu S, Ding H, Su F, Zhang A, Jiang M (2009).Involvement of protein phosphorylation in water stress-induced antioxidant defense in maize leaves. J. Integr. Plant Biol. 51(7), 654-662

Key words: abscisic acid, antioxidant defense, hydrogen peroxide, protein phosphorylation, water stress.

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